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- Table of Contents
SLFN14 / Protein SLFN14 · Western blot design guide
Design a Western Blot for SLFN14
Source-linked SLFN14 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-SLFN14 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled October 2026 · For research use; verify linked source records and product datasheet before use
SLFN14 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~103.9 kDa | |
| Observed band | 104 kDa | |
| Gel | 8% (catalog A11813-1) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | — | |
| Regulation | Not found in UniProt | |
| Isoform | 2 isoform(s) |
Section 1
Source-Linked SLFN14 Western Blot Protocol Options
The A11813-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human HEL, rat liver, mouse liver (catalog A11813-1) |
| Gel % | 8% (catalog A11813-1) |
| Load | 30 ug; reducing conditions (catalog A11813-1) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A11813-1) |
| Membrane | nitrocellulose membrane (catalog A11813-1) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A11813-1) |
| Primary antibody | A11813-1 · 0.5 μg/mL (catalog A11813-1) |
| Primary incubation | overnight at 4°C (catalog A11813-1) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A11813-1) |
| Secondary incubation | 1.5 hour at RT (catalog A11813-1) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A11813-1) |
| Detection | ECL (catalog A11813-1) |
Section 2
What Is the Expected SLFN14 Western Blot Band Size?
SLFN14 is predicted at 103.9 kDa and observed near 104 kDa; this close agreement needs no additional migration explanation.
| Band near 104 kDa | matches the 104 kDa empirical SLFN14 band |
| Band near 104 kDa in whole-cell lysate | matches the reported signal in HEL whole-cell lysate |
| Additional bands at other sizes | could reflect isoforms 1 and 2; their migration is unknown |
| Weak signal in a nuclear-depleted fraction | is consistent with SLFN14's nuclear localization |
💡Expected SLFN14 appearanceSLFN14 has a predicted mass of 103.9 kDa and an empirical band near 104 kDa; confirm band identity with appropriate antibody controls.
| Predicted mass | places the reference band near 103.9 kDa; the reported band is 104 kDa |
| Alternative splicing | produces isoforms 1 and 2, whose relative migration is unspecified |
| Isoform 1 | has no supplied isoform-specific mass or migration |
| Isoform 2 | has no supplied isoform-specific mass or migration |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | SLFN14 is nuclear and may be poorly recovered | check nuclear extraction and a positive lysate control |
| Band higher than expected | no supplied modification explains a larger SLFN14 band | compare with the 104 kDa control band and check antibody specificity |
| Band lower than expected | isoform migration is unknown; a truncated SLFN14 form is described | check antibody specificity and compare antibodies against different regions |
| Multiple bands | isoforms 1 and 2 exist, but distinct bands are unverified | confirm candidate bands with an independent antibody or SLFN14 depletion |
| Weak or no signal | nuclear SLFN14 may be underrepresented in the sample | test a nuclear-enriched fraction alongside a positive control |
Experiment evidence
Western blot images showing SLFN14 band
A11813-1 · SLFN14
A11813 · SLFN14
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for SLFN14 Western blot
🧪For positive controls for SLFN14 in Western blot, you can use an independently validated SLFN14-positive sample, since HPA provides no positive tissue or cell candidate.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA provides no expression data, so suitable tissue controls require independent validation.
HPA tissue expression evidence for SLFN14
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced SLFN14 Western Blot Tips
Deeper troubleshooting and optimisation questions for SLFN14, answered from its protein features.
How should SLFN14 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could SLFN14 isoforms produce different bands?
Isoforms · UniProt lists two isoforms. Isoform 2 lacks residues 353–368 in UniProt canonical numbering, so it may migrate differently. The supplied features do not establish whether the isoforms resolve into separate bands.
Can an annotated modification explain an SLFN14 band shift?
PTM · The supplied features list no modified residues or glycosylation sites. Do not assign a shifted band to a specific modification from these data alone.
Does this guide establish induction of SLFN14?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for SLFN14 Western blot?
Transfer · SLFN14 is predicted at 103.9 kDa, with an apparent band at 104 kDa. Check that your transfer retains detectable protein around 104 kDa; the supplied features do not specify a transfer method.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A11813-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should I quantify SLFN14 bands?
Quantitation · Measure the band near the supplied 104 kDa apparent position consistently across samples. If additional bands appear, report them separately unless their identity as SLFN14 is established.
Why does SLFN14 appear near 104 kDa?
Interpretation · The supplied apparent band is 104 kDa, close to SLFN14’s predicted 103.9 kDa. This agreement does not establish which isoform is present or whether the band contains modified protein.
Isoform 2 lacks canonical residues 353–368. UniProt also describes a C-terminally truncated SLFN14 endoribonuclease that associates with ribosomes independently of ATP. Neither feature alone identifies a smaller band; consider antibody recognition and band identity before assigning it.
UniProt places protein SLFN14 in the nucleus. A nuclear fraction is therefore relevant when checking localization, but these features do not establish how much SLFN14 should appear in any particular sample.
Isoform 2 lacks canonical residues 353–368. UniProt also describes a C-terminally truncated SLFN14 endoribonuclease that associates with ribosomes independently of ATP. Neither feature alone identifies a smaller band; consider antibody recognition and band identity before assigning it.
UniProt places protein SLFN14 in the nucleus. A nuclear fraction is therefore relevant when checking localization, but these features do not establish how much SLFN14 should appear in any particular sample.
Boster reagents
SLFN14 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-SLFN14 Antibody Picoband®
Cat # A11813-1
Real WB data
Anti-Protein SLFN14 SLFN14 Antibody
Cat # A11813
Two the supplier anti-SLFN14 antibodies have WB images: A11813-1 shows an approximately 104 kDa band in human HEL, rat liver, and mouse liver lysates; A11813 is shown with mouse kidney lysate. These examples document specific tested samples, not broader validation.
Which to pick: Choose A11813-1 for the documented HEL or liver examples; it lists human, mouse, and rat reactivity. Choose A11813 for the documented mouse kidney example at 1 or 2 μg/mL; it lists human and mouse reactivity.
Source: BosterBio SLFN14 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P0C7P3.
- Human Protein Atlas. SLFN14 tissue expression.
- PMC5990420 — target-verified WB comparison
- PMC11054720 — target-verified WB comparison
- PMC6004054 — target-verified WB comparison