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Product Info Summary
| SKU: | A11813 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Protein SLFN14 SLFN14 Antibody
SKU/Catalog Number
A11813
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein SLFN14 SLFN14 Antibody (Catalog # A11813). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
SLFN14 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein SLFN14 SLFN14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11813)
Host
Rabbit
Contents
SLFN14 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SLFN14 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus human SLFN14. The immunogen is located within amino acids 730 - 780 of SLFN14.
Reactive Species
A11813 is reactive to SLFN14 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
103.9 kDa
Background of SLFN14
Despite being first described several years ago, the roles of the Schlafen (SLFN) family of proteins remain largely unknown. The SLFN genes are preferentially expressed in lymphoid tissues and differentially regulated during thymocyte maturation. It is thought that many play roles in cell growth, hemopoietic cell differentiation, and T cell development and maturation. Most members contain at least one divergent AAA domain (AAA_4) that may play a role in ATP binding. SLFN14 is a recently identified member of the SLFN family; its role has yet to be determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11813 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SLFN14 antibody can be used for detection of SLFN14 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SLFN14 in mouse kidney tissue lysate with SLFN14 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of SLFN14 in mouse kidney tissue with SLFN14 antibody at 20 μg/mL.
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Th17/Treg cell differentiation ratio and TWEAK/Fn14 signaling level in AC mice. ( A ) The state of mice ocular surface was observed by slit-lamp. ( B ) The clinical scores of mice were performed according to the status of eyelid, conjunctiva and cornea. ( C ) HE staining was utilized to evaluate the pathological changes of conjunctival tissue in mice. ( D ) The proportion of Th17 or Treg cells in spleen of mice was assessed by flow cytometry. ( E ) The levels of Th17 and Treg cytokines in mice spleen were evaluated by ELISA. ( F-H ) TWEAK and Fn14 levels in ocular conjunctival tissue were measured by qRT-PCR and WB. ( I ) IHC was used to observe the protein level of TWEAK and Fn14 in ocular conjunctival tissue. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. Control
Index in PubMed under a CC BY license. PMID: 39592944
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TWEAK knockdown affected conjunctivitis in AC mice. ( A ) The effect of TWEAK knockdown on the state of mice ocular surface was observed under slit-lamp. ( B ) Clinical score of eyelid, conjunctiva and cornea to assess the effect of TWEAK knockdown in AC mice. ( C-D ) HE staining and TB staining were utilized to evaluate the effect of TWEAK knockdown on conjunctivitis in mice. ( E-G ) The levels of TWEAK and Fn14 in conjunctival tissue were detected by qRT-PCR, IHC and WB. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. Con + AAV-shNC; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. AC + AAV-shNC; ns, no significant difference
Index in PubMed under a CC BY license. PMID: 39592944
Specific Publications For Anti-Protein SLFN14 SLFN14 Antibody (A11813)
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