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- Table of Contents
Facts about Transcription factor Sp2.
Human | |
---|---|
Gene Name: | SP2 |
Uniprot: | Q02086 |
Entrez: | 6668 |
Belongs to: |
---|
Sp1 C2H2-type zinc-finger protein family |
KIAA0048; Sp2 transcription factor; transcription factor Sp2
Mass (kDA):
64.9 kDA
Human | |
---|---|
Location: | 17q21.32 |
Sequence: | 17; NC_000017.11 (47896032..47932271) |
Nucleus.
The SP2 marker is a transcription factor, a member of the Sp family. It is a protein that is found in the genome of human cells. Scientists who are involved in research can submit their findings and receive product credits. This technology is applicable to scientists around the world. It can be used to test for Sp2 levels in various samples. However, some people may be confused on how to use the SP2 marker. This article will help you understand the SP2 marker and how it works.
The SP2 gene codes for a transcription factor of the SP family and the XKLF family. Sp family members are sequence-specific DNA-binding proteins that bind to target genes and promote gene expression. Sp2 protein is the least conserved among these proteins and localizes to subnuclear foci associated with the nuclear matrix. It is a transcription factor that activates expression from a variety of promoters. Antibodies to Sp2 have been produced for several applications, and these antibodies have been validated in WB and IHC for human and mouse use.
The SP2 Cell-Based ELISA kit can monitor the SP2 protein expression profile in cultured cells and measure the relative amounts of SP2 in cultured cells. It is an excellent tool for screening the effects of treatment and activators on SP2 expression. However, you can buy individual antibodies to this protein. Hence, the best way to use this marker is by comparing your results with those of other scientists' results.
The SP2 gene encodes a transcription factor that binds DNA. This gene is part of the Sp subfamily and the XKLF transcription factor family. Sp family proteins are sequence-specific DNA-binding proteins that can activate expression from a variety of promoters. Boster Bio has validated the SP2 marker with its antibodies on WB and IHC, as well as ELISA.
The SP2 marker is a member of the Cys2-His2 family and is a transactivator. It has a highly conserved carboxyl-terminal DNA-binding domain with three zinc fingers. The Sp2 protein family includes nine members in mammals. It also has a trans-activation domain at its amino-terminal end. Trans-activation is dependent on post-translational modifications, interaction with other Sp proteins, and other components of the basal transcription complex.
The Sp2 marker is a transcription factor found in basal keratinocytes. Overexpression of Sp2 inhibits keratinocyte differentiation and increases the sensitivity to a number of carcinogens. In addition, expression of Sp2 increases with age. Hence, the SP2 marker may be of interest in the study of keratinocyte differentiation. The SP2 marker has potential as a diagnostic tool.
To study the effect of Sp2 knockdown on RFX1-8 gene expression, we performed a transgene integration assay in mouse embryos. The results showed that the SP2 marker has a significant effect on RFX gene expression. We further determined whether RFX genes are functionally dependent on Sp2 in mouse embryos. Using this transgene, we were able to identify a functional upstream regulatory gene for RFX1-8.
This cell-based ELISA kit is a convenient, high-throughput way to measure protein phosphorylation in cells. The results are highly sensitive and can be used to monitor the effects of activators and inhibitors, and to determine the effect of treatments on protein phosphorylation. This kit requires seeding cells on poly-l-lysine coated plates.
The SP2 colorimetric cell-based ELISA kit is a convenient, high-throughput method to determine the concentration of SP2 in cultured cells. It can be used to determine the relative amounts of SP2 in different types of cells. This technique can be used to monitor the effects of different treatment or activators on SP2 expression in cells. Aside from measuring protein levels, it also measures phosphorylation and post-translational modifications on SP2.
CeIl-ELISA detects antibodies against fixed cells of MCF7 breast carcinoma, MDA-MB-468 human fibroblast, and MCF10-2A normal breast epithelium. A negative control of the kit is provided as irrelevant anti-SP2 antibody. The test was conducted against the scFvEC23 library, which contains an anti-SP2 antibody gene.
The Boster Bio SP2 Marker is essentially a primary antibody. This antibody has been produced with high affinity and is validated for use in ELISA, Western Blotting and Immunohistochemistry. This antibody has been validated for the detection of a wide variety of proteins in a range of tissues. Specifically, it is designed for the detection of the human immunoglobulin G (IgG).
This system enables the use of two antibodies on the same specimen, resulting in dual labeling. Dual labeling enables more questions to be answered by a single antibody and gives researchers more contextual data to analyze. It is ideal for cellular biology and immunohistochemistry. For these purposes, a primary antibody and secondary antibody are both required. Using a primary antibody or secondary antibody in conjunction with a secondary antibody allows researchers to create more accurate and robust answers.
Boster Bio is an antibody manufacturer that specializes in western blotting and immunohistochemistry compatible antibodies. The company also provides services such as custom mouse monoclonal antibodies and recombinant protein expression. It also offers multiplex cytokine assay services. Their products are manufactured in-house, and they meet the highest quality standards. The company also maintains strict standards and ensures efficiency. Its extensive range of products is ideal for a variety of research applications.
Boster Bio's products are used by researchers and industry professionals alike. Boster Bio antibodies are optimized for IHC, WB, and ELISA. The company's products have received more than 29,000 citations and are validated for IHC, WB, and Flow Cytometry. You can be confident that every Boster Bio antibody will work as advertised. You can purchase the antibodies directly from Boster Bio.
Immunohistochemistry is a special process for staining cell tissues to identify antigens or haptens. Boster Bio uses high-throughput screening techniques to optimize antibody dilutions for use in IHC. You can order multiplex kits to detect multiple targets, allowing for high throughput during screening and fast assay processing. Boster Bio's Picokine ELISA kits contain sufficient reagents for 96 tests.
PMID: 1341900 by Kingsley C., et al. Cloning of GT box-binding proteins: a novel Sp1 multigene family regulating T-cell receptor gene expression.