Click image to see more details
Product Info Summary
| SKU: | A02284 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Transcription factor Sp2 SP2 Antibody
SKU/Catalog Number
A02284
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Transcription factor Sp2 SP2 Antibody (Catalog# A02284). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transcription factor Sp2 SP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02284)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human SP2. Immunogen sequence location: 471-520
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02284 is reactive to SP2 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
64.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02284 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Click image to see more details
Western blot analysis of the lysates from 293 cells using SP2 antibody.
Click image to see more details
Western blot analysis of lysates from Jurkat cells, using SP2 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Transcription factor Sp2 SP2 Antibody (A02284)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Transcription factor Sp2 SP2 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Transcription factor Sp2 SP2 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-Transcription factor Sp2 SP2 Antibody
Question
I was wanting to use your anti-SP2 antibody for WB for human bone marrow on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human bone marrow identification?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
You can see on the product datasheet, A02284 anti-SP2 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human bone marrow in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-08
Question
I would like to test anti-SP2 antibody A02284 on human bone marrow for research purposes, then I may be interested in using anti-SP2 antibody A02284 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
The products we sell, including anti-SP2 antibody A02284, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-28
Question
I have attached the WB image, lot number and protocol we used for bone marrow using anti-SP2 antibody A02284. Please let me know if you require anything else.
A. Zhang
Verified customer
Asked: 2015-06-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-06-19
Question
Is a blocking peptide available for product anti-SP2 antibody (A02284)?
M. Moore
Verified customer
Asked: 2013-02-07
Answer
We do provide the blocking peptide for product anti-SP2 antibody (A02284). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2013-02-07
