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- Table of Contents
SRR / Serine racemase · Western blot design guide
Design a Western Blot for SRR
Real validated SRR Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-SRR WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
SRR Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~36.6 kDa | |
| Observed band | ~37 kDa | |
| Gel | 10% (catalog A02660-2) | |
| Positive control | Colon (IHC candidate; verify WB) +4 more | |
| Negative control | Oral mucosa (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | — | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Real Curated SRR Western Blot Protocols
The A02660-2 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human U251, human SiHa, human SH-SY5Y (catalog A02660-2) |
| Gel % | 10% (catalog A02660-2) |
| Load | 30 ug; reducing conditions (catalog A02660-2) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A02660-2) |
| Membrane | nitrocellulose membrane (catalog A02660-2) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A02660-2) |
| Primary antibody | A02660-2 · 0.5 μg/mL (catalog A02660-2) |
| Primary incubation | overnight at 4°C (catalog A02660-2) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A02660-2) |
| Secondary incubation | 1.5 hour at RT (catalog A02660-2) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A02660-2) |
| Detection | ECL (catalog A02660-2) |
Section 2
What Is the Expected SRR Western Blot Band Size?
SRR is predicted at 36.6 kDa and observed at ~37 kDa; the cause of the small difference is not established.
| Band at ~37 kDa | Empirical SRR band, close to the 36.6 kDa predicted monomer mass |
| Band near ~74 kDa | Possible SRR homodimer if it survives sample preparation; confirm its identity |
| ~74 kDa band disappears after stronger denaturation | Consistent with dissociation of a retained SRR homodimer |
| Single ~37 kDa band despite modified residues | The listed modifications do not establish a resolvable size shift |
💡Expected SRR appearanceSRR has a predicted mass of 36.6 kDa and an empirical band at ~37 kDa; confirm band identity with appropriate antibody and sample controls.
| Predicted SRR monomer mass | 36.6 kDa predicted, with an empirical band at ~37 kDa |
| Homodimer formation | Could produce a band near twice the monomer size if the dimer survives preparation |
| N6-(pyridoxal phosphate)lysine at residue 56 | No visible migration change is established |
| S-nitrosocysteine at residue 113 | No visible migration change is established |
| Situation | Likely cause | Next action |
|---|---|---|
| Band higher than expected | SRR homodimer may persist during sample preparation | Compare fully denatured samples and verify the band with an independent antibody |
| Multiple bands | A retained homodimer or nonspecific antibody binding | Compare denaturation conditions and use an independent antibody |
| Band lower than expected | Possible sample degradation; no smaller SRR product is specified | Use fresh lysate with protease inhibitors and confirm band identity |
| No band in lysate | Low SRR abundance or inadequate detection | Check loading and transfer with a positive-control lysate |
| Weak or no signal | Insufficient target or antibody sensitivity | Increase lysate input and check antibody performance with a positive control |
| Fragments below expected size | Possible proteolysis during sample handling | Prepare fresh lysate with protease inhibitors and verify fragments independently |
Experiment evidence
Western blot images showing SRR band
A02660-2 · SRR
A02660-2 · SRR
M02660-1 · SRR
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for SRR Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for SRR in Western blot, you can use colon tissue, which HPA scores high for SRR.
Positive control: Colon (IHC candidate; verify WB)
Negative control: Oral mucosa (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA identifies a high-expression tissue and a not-detected tissue, so tissue controls are feasible.
HPA tissue expression evidence for SRR
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Colon | endothelial cells | High | Protein (IHC) | HPA → |
| Adipose tissue | adipocytes | Medium | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Medium | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Medium | Protein (IHC) | HPA → |
| Bronchus | respiratory epithelial cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Oral mucosa | squamous epithelial cells | Not detected | Protein (IHC) | HPA → |
| Ovary | ovarian stroma cells | Not detected | Protein (IHC) | HPA → |
| Smooth muscle | smooth muscle cells | Not detected | Protein (IHC) | HPA → |
| Spleen | cells in red pulp | Not detected | Protein (IHC) | HPA → |
| Vagina | squamous epithelial cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced SRR Western Blot Tips
Deeper troubleshooting and optimisation questions for SRR, answered from its protein features.
How should SRR band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could SRR isoforms explain multiple bands?
Isoforms · The supplied UniProt record lists one isoform and no alternative sequence. It does not support assigning additional bands to SRR isoforms.
Which SRR modifications should I consider when interpreting bands?
PTM · UniProt lists N6-(pyridoxal phosphate)lysine at position 56 and S-nitrosocysteine at position 113. These are UniProt coordinates; paper or antibody numbering may differ. Their presence does not establish a visible band shift.
The supplied features list no glycosylation sites, signal peptide, or propeptide. They therefore provide no basis to assign a second band to glycosylation or cleavage of those peptides.
The supplied features list no glycosylation sites, signal peptide, or propeptide. They therefore provide no basis to assign a second band to glycosylation or cleavage of those peptides.
Does this guide establish induction of SRR?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for SRR?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A02660-2 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
Which band should I quantify for SRR?
Quantitation · Quantify the consistently identified band near the observed ~37 kDa position, using the same band boundary across samples. The predicted mass is 36.6 kDa; the supplied features do not establish the identity of other bands.
Why is the SRR band near 37 kDa?
Interpretation · SRR has a predicted mass of 36.6 kDa, close to the observed ~37 kDa band. That agreement supports the assignment, but apparent mass alone does not confirm identity.
UniProt identifies S-nitrosocysteine at position 113, using UniProt numbering. This feature alone cannot establish that a shifted band is nitrosylated SRR or that the modification changes apparent mass.
UniProt describes SRR as a homodimer. A higher band could prompt investigation of oligomerization, but the homodimer annotation alone does not show that a dimer survives Western blot sample preparation.
UniProt identifies S-nitrosocysteine at position 113, using UniProt numbering. This feature alone cannot establish that a shifted band is nitrosylated SRR or that the modification changes apparent mass.
UniProt describes SRR as a homodimer. A higher band could prompt investigation of oligomerization, but the homodimer annotation alone does not show that a dimer survives Western blot sample preparation.
Boster reagents
SRR Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Serine racemase/SRR Antibody Picoband®
Cat # A02660-2
Real WB data
Anti-Serine racemase Rabbit Monoclonal Antibody
Cat # M02660-1
Two the supplier anti-SRR antibodies have Western blot images. A02660-2 shows an approximately 37 kDa band in the listed human cell and rodent brain lysates. M02660-1 shows blots of 293 and U-87 MG cell lysates, with limited conditions reported.
Which to pick: Choose A02660-2 for human, mouse, or rat samples when the documented 37 kDa band and detailed blot conditions are useful. M02660-1 is listed for human samples and has a blot image from 293 and U-87 MG lysates.
Source: BosterBio SRR gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q9GZT4.
- Human Protein Atlas. SRR tissue expression.
- PMC9305835 — target-verified WB comparison