Anti-Serine racemase/SRR Antibody Picoband™

Product Name

Anti-Serine racemase/SRR Antibody Picoband™

View all Serine racemase Antibodies

SKU/Catalog Number

A02660-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Serine racemase/SRR Antibody Picoband™ catalog # A02660-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Monkey.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Serine racemase/SRR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02660-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Serine racemase/SRR recombinant protein (Position: A3-E283).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02660-2 is reactive to Srr in Human, Monkey

Applications

A02660-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

37 kDa

Calculated molecular weight

36.359kDa

Background of Serine racemase

SRR(serine racemase) is a 37 kDa protein and belongs to the serine/threonine dehydratase family. It plays a role in neuronal activity and a potential biological role in the regulation of N-methyl-D-aspartate (NMDA) receptor activity in these peripheral organs as well. SRR catalyzes the racemization between l- and d-serine.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Monkey
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Serine racemase/SRR using anti-Serine racemase/SRR antibody (A02660-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Serine racemase/SRR antigen affinity purified polyclonal antibody (Catalog # A02660-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Serine racemase/SRR at approximately 37 kDa. The expected band size for Serine racemase/SRR is at 37 kDa.

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Figure 2. Flow Cytometry analysis of U87 cells using anti-Serine racemase/SRR antibody (A02660-2).
Overlay histogram showing U87 cells stained with A02660-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Serine racemase/SRR Antibody (A02660-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For Srr (Source: Uniprot.org, NCBI)

Alternative Names

D-serine ammonia-lyase; D-serine dehydratase; EC 4.3.1.17; EC 4.3.1.18; EC 5.1.1.18; ILV1; ISO1; L-serine ammonia-lyase; L-serine dehydratase; serine racemase Srr|M100034, Rgsc, Rgsc34, Srs|serine racemase|serine racemase|D-serine ammonia-lyase|D-serine dehydratase|L-serine ammonia-lyase|L-serine dehydratase

For more info on Srr, check out the Srr Infographic

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In this infographic, you will see the following information for Srr: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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