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We validate the specificity of these antibodies to Hsc70-interacting protein by testing them on tissues known to express ST13 positively and negatively. Browse below to find the ST13 antibody that suites your experiment. We have 7 of these antibodies and many publications and validation images.
If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.
Facts about Hsc70-interacting protein.
Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins (By similarity). .
AAG2; aging-associated protein 2; FAM10A1FAM10A4; heat shock 70kD protein binding protein; Hip; HIPFLJ27260; HOP; hsc70-interacting protein; Hsp70-interacting protein; HSPABP1; P48MGC129952; PRO0786; Progesterone receptor-associated p48 protein; Protein FAM10A1; Putative tumor suppressor ST13; Renal carcinoma antigen NY-REN-33; SNC6HSPABP; suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein); suppression of tumorigenicity 13 (colon carcinoma) (Hsp70-interacting protein); Suppression of tumorigenicity 13 protein
|Sequence:||22; NC_000022.11 (40824535..40857008, complement)|
If you are looking for high-affinity prim antibodies such as ST13, it might be helpful to optimize your experiments. The boster Bio Optimization Guide will guide you through all of the options and answer any queries you may have. Flow procedures offer many options so it is important that you have a guide to help you optimize your experiments. Boster Bio's website has more information about optimizing experiments.
Flowcytometry is a highly useful technique that allows researchers and doctors to determine the number, distribution, and fate of cancer cells. This requires the use of cells or particles as samples. Antibodies can be used to detect these particles. Boster Bio offers monoclonal as well as polyclonal primary antibodies that are high-affinity against the ST13 marker. These antibodies are well-known for their long-term use and have received many citations in scientific journals.
Monoclonal antigens are IgG antibodies that recognize a single epitope (or Fab fragment) on a particular type of antigen. They are generally less affinity and have lower background, cross-reactivity, protein stability, and greater affinity than other IgG molecules. These antibodies can be difficult to purify. They should be applied in concentrated form, and must go through rigorous washing without disrupting the specific binding. Unlike polyclonal antibodies, monoclonal antibodies are more specific to specific peptide epitopes.
High-affinity primary antibodies should bind a specific antibody with a high affinity to achieve optimal results. The optimal antibody for immunocytochemistry should also bind to the target antigen in a high dilution, ensuring that nonspecific interactions are eliminated. As short incubation times can lead to signal attenuation, the optimal antibody should also bind the target antigen at a high affinity.
As stated previously, affinity refers to the strength of binding between one molecule and a bimolecular-ligand molecule. The equilibrium constant (KD), measures affinity in bimolecular relationships. The rate at which an affinity binding agent can be detected is proportional to its concentration. It is equal to its rate of dissociation. KD values are determined by the proportion of reactants which are often antibodies.
Antibodies are valuable resources for research. The author should document and describe them correctly. Inadequate documentation regarding the control can lead both to mistakes and rejections of scientific work. Because the ST13 marker allows researchers and scientists to identify proteins with high affinity, it is extremely important. If the staining results look similar to those of a single immunoglobulin monoclonal antibody, then it is possible that antibodies were raised against a particular antigen.
High-affinity primaries antibodies were previously able to detect proteins even at low concentrations. This isn't always true. Because of its low affinity for biotin, a low-affinity primary antibody could be mislabeled. The same goes for unlabeled antibodies.
There are several benefits to antipeptides antibodies over monoclonal. They have high binding affinity but low affinity for the antigen. Monoclonal antibodies, on the contrary, are monoclonal. They recognize antigens smaller than one antibody can recognize. Monoclonal antibodies can also be made from a DNA sequence.
Because antibodies can be used for many applications, multiple approaches are necessary to validate their specificity. Multiple approaches are needed to confirm the specificity of an antibody, but each approach must be evaluated by its "weight of evidence."
There are many options for optimizing your Boster Bio ordering. Boster pipettes have the ability to disperse aqueous solutions from 0.5 ul until 1 ml. Multichannel pipettes may be more useful if you plan to dispense large volumes of samples. If you plan to validate your results and receive product credits, you can share them.