Anti-HSC70 Interacting Protein HIP/ST13 Antibody Picoband®

Product Name

Anti-HSC70 Interacting Protein HIP/ST13 Antibody Picoband®

View all ST13 Antibodies

SKU/Catalog Number

PA1935
BA2857 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HSC70 Interacting Protein HIP/ST13 Antibody catalog # PA1935. Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HSC70 Interacting Protein HIP/ST13 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1935)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ST13, different from the related rat and mouse sequences by two amino acids.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1935 is reactive to ST13 in Human

Observed Molecular Weight

45-54 kDa

Calculated molecular weight

41.3 kDa

Background of ST13

ST13 (Suppression of Tumorigenicity 13), also known as P48 or HIP, is a protein that in humans is encoded by the ST13 gene. ST13 is an abundant, highly conserved protein that binds the major cytosolic chaperones heat-shock protein 70-kD (HSP70) and HSP90 during an intermediate stage of steroid receptor assembly, but is absent from the mature receptor complex. Zhang et al. (1998) mapped the ST13 gene to chromosome 22q13 by fluorescence in situ hybridization. They noted that colorectal, breast, and ovarian carcinomas frequently show loss of heterozygosity at this site. Using a yeast 2-hybrid assay, Hohfeld et al. (1995) showed that rat Hip bound Hsc70 (HSPA8). One Hip oligomer bound the ATPase domains of at least 2 Hsc70 molecules, and binding was dependent on activation of the Hsc70 ATPase by Hsp40 (DNAJB1). Hip stabilized the ADP-bound form of Hsc70, which had a high affinity for a test protein substrate. Hohfeld et al. (1995) concluded that HIP contributes to interactions of HSC70 with target proteins through its own chaperone activity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1935 is guaranteed for IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human

Positive Control

WB: human Hela whole cell, human 293T whole cell, human SKOV3 whole cell, mouse testis tissue, mouse NIH/3T3 whole cell
ICC/IF: Hela cell

Validation Images & Assay Conditions

See full target information ST13

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