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Product Info Summary
|Description:||Transient overexpression lysate of suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein) (ST13)|
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ST13 (NM_003932) Human Over-expression Lysate
Transient overexpression lysate of suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein) (ST13)
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Cite This Product
ST13 (NM_003932) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006767)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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ST13 Human Over-expression Lysates NM_003932.
Western blot validation of overexpression lysate (LS006767) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ST13 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for ST13 (Source: Uniprot.org, NCBI)
AAG2; aging-associated protein 2; FAM10A1FAM10A4; heat shock 70kD protein binding protein; Hip; HIPFLJ27260; HOP; hsc70-interacting protein; Hsp70-interacting protein; HSPABP1; P48MGC129952; PRO0786; Progesterone receptor-associated p48 protein; Protein FAM10A1; Putative tumor suppressor ST13; Renal carcinoma antigen NY-REN-33; SNC6HSPABP; suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein); suppression of tumorigenicity 13 (colon carcinoma) (Hsp70-interacting protein); Suppression of tumorigenicity 13 protein ST13 AAG2, FAM10A1, FAM10A4, HIP, HOP, HSPABP, HSPABP1, P48, PRO0786, SNC6 ST13 Hsp70 interacting protein hsc70-interacting protein|Hsp70-interacting protein|aging-associated protein 2|heat shock 70kD protein binding protein|progesterone receptor-associated p48 protein|putative tumor suppressor ST13|renal carcinoma antigen NY-REN-33|suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein)|suppression of tumorigenicity 13 protein|testis secretory sperm-binding protein Li 233m*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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