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Facts about Stromal interaction molecule 1.
Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores (PubMed:15866891, PubMed:16005298, PubMed:16208375, PubMed:16537481, PubMed:16733527, PubMed:16766533, PubMed:16807233, PubMed:18854159, PubMed:19249086, PubMed:22464749, PubMed:24069340, PubMed:24351972, PubMed:24591628, PubMed:26322679, PubMed:25326555, PubMed:28219928).
Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domainname.Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit ORAI1 (PubMed:16208375, PubMed:16537481). Involved in enamel formation (PubMed:24621671).
Human | |
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Gene Name: | STIM1 |
Uniprot: | Q13586 |
Entrez: | 6786 |
Belongs to: |
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No superfamily |
GOK; GOKD11S4896E; STIM1; stromal interaction molecule 1
Mass (kDA):
77.423 kDA
Human | |
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Location: | 11p15.4 |
Sequence: | 11; NC_000011.10 (3855664..4093210) |
Ubiquitously expressed in various human primary cells and tumor cell lines.
Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Cytoplasm, cytoskeleton. Sarcoplasmic reticulum. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium and is detected at punctae corresponding to junctions between the endoplasmic reticulum and the cell membrane (PubMed:19249086, PubMed:16005298, PubMed:16208375, PubMed:18854159). Associated with the microtubule network at the growing distal tip of microtubules (PubMed:19632184). Colocalizes with ORAI1 at the ce
In this article, you'll learn more about Steven Boster and historial background. You will learn about the best use of this marker in immunohistochemistry, ELISA kits, and antibodies. Every scientist in the world benefit from his research and earn Boster Rewards. Anyone curious about learning more about the STIM1 marker can take part in this reward program. It can be used to create your own antibodies.
Boster bio's Historial background is a crucial element of the science and art of microbial biology. Boster bios are a method by which scientists can report results from studies on specific species or applications to be eligible for product credits. The idea of Historial background in Boster bio is applicable to scientists of all levels across the globe. There are many reasons behind this concept. For more information, read the following biography of Steven Boster, founder of Boster Bio.
Antibodies based on the STIM1 marker were created to detect STIM1 expression. This marker was expressed in human cells. This experiment used mouse anti-STIM1 antibody (1:100) and normal goat serum (1,100). The primary antibodies were then followed by appropriate secondary antibodies. Antibodies using the STIM1 marker were stained using the avidin-biotin-peroxidase (ABP) complex method.
In addition to being a key transcription factor in human cells, STIM1 is also involved in various cellular processes, such as muscle contraction, the release of hormones and neurotransmitters and gene transcription. STIM1 recently received more attention due to its involvement in the oncogenic process. It has been found to inhibit cell invasion and migration and also to inhibit proliferation in various cancer models. Despite its significance in human biology, it is not known what is the exact mechanism of action is.
We tested a combination of antistim1 and anti-CD31 antibodies to determine if these antibodies target the STIM1 gene. This combination stopped the proliferation of EBV-infected NPC cells. On day 30, xenografts taken from mice that had been killed were removed. We weighed the xenografts in the morning and after staining with anti-STIM1 antibody and b-actin antibody to determine their expression.
STIM1 also has a connection with HCC, and other types of cancers. It is a major initiator of a molecule in SOCE. It is also found in the ER membrane with Orai1.
The Boster Bio STIM1 ELISA kit is an extremely sensitive test for the TGF-b1 protein and Col-I/III, two proteins that regulate cell growth and survival. The kit measures absorption of TGF-b1 at 450nm in order to determine the amount in the sample. Its sensitivity and reactivity are also extremely stable. This kit is available to all scientists around the world.
The Human Epithelialstromal Interaction Protein 1 (STIM1) ELISA Kit is used to determine levels in serum, plasma and tissue homogenates. You can also purchase additional reagents in five separate kits. The kit's cost-efficient five-plate, 96-well design makes it simple to use and a cost-effective choice for scientists and researchers.
The kit contains anti-Orai1 antibodies to detect STIM1 in blood samples and excretion body. The kit also contains an commercially available sample. Boster Bio's STIM1 ELISA Kit is sensitive and can be used to test STIM1 levels in blood samples. Additionally, the kit can be used to determine the levels of STIM1 in peripheral blood.
STIM1 is an indicator of a cellular protein that is derived from senescence-derived fibrocytes. STIM1 is located in a clump at the SR-PM junction in living. Its location may change in response to KO. This question can be answered by looking at STIM1 clusters in the same levels. Further, reference to STIM1 clusters in eukaryotic cells are necessary to determine its function and the regulation.
The STIM1 protein comprises 0.1-0.9 percent of total protein. It can be as high as 90 percent of the total. However, individual data points are more than 1.0 of the total protein. The protein is not present in peripheral ER/PM contacts. Therefore, it is important to discover the molecular mechanism which governs the connection of the STIM1 marker with the SR and PM.
STIM1 is present in the prostate, brain, and in the cervix, which is a part of the human body. It has been established that this marker is associated with the amount of STIM1 found in tumors. Its expression is high in cervical cancer. It has been found in the tissues of patients as shown in Figure.
Formerly known as GOK, STIM1 (90-kDa transmembrane protein of type 1) senses the loss of calcium and communicates with the Ca2+ channel in the plasma membrane Orai1. The extracellular/lumenal domain of STIM-1 contains a EF-hand Ca2+-binding motif. In addition the human STIM1 protein has a SAM multimerization domain.
STIM1 has not yet been proven to be present in smooth muscle cells, but it is possible to discuss this further. STIM1 is likely to be located close to the plasma and sarcoplasmic-reticulum membranes, and it could be located in close proximity to proteins associated with smooth muscle cells. It may also be bound to a different protein that is located at the PM. This protein could be another type Ca2+ channel that is L-type, or TRP channel.
PMID: 8921403 by Parker N.J., et al. Molecular cloning of a novel human gene (D11S4896E) at chromosomal region 11p15.5.
PMID: 9377559 by Sabbioni S., et al. GOK: a gene at 11p15 involved in rhabdomyosarcoma and rhabdoid tumor development.
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