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SULT1A1 / Sulfotransferase 1A1 · Western blot design guide
Design a Western Blot for SULT1A1
Source-linked SULT1A1 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-SULT1A1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
SULT1A1 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~34.2 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Small intestine (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated | |
| Caveat | Phosphorylation-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 2 isoform(s) |
Section 1
Source-Linked SULT1A1 Western Blot Protocol Options
The A03500-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | lysates from HepG2 cell line and human lung tissue (from left to right), (catalog A03500-1) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A03500-1; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | goat anti-rabbit IgG, 1:10000 (catalog A03500-1) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected SULT1A1 Western Blot Band Size?
SULT1A1 has a predicted 34.2 kDa monomer; isoforms and homodimerization could affect the pattern, but distinct migration has not been demonstrated here.
| Single band near 34.2 kDa | Consistent with the predicted SULT1A1 monomer; isoforms may comigrate. |
| Band near 68 kDa | Could reflect the annotated homodimer if it survives electrophoresis; confirm its identity. |
| Two bands in the monomer region | Could reflect isoforms 1 and 2 if they migrate differently; confirm their identities. |
| Monomer and higher band together | Could reflect both dissociated SULT1A1 and retained homodimer; confirm both bands. |
💡Expected SULT1A1 appearanceThe predicted monomer mass is 34.2 kDa; isoforms or retained homodimers could alter the pattern, so confirm any band with ordinary identity controls.
| Predicted monomer mass | Provides a 34.2 kDa reference for the expected band. |
| Homodimer | Could produce a band near twice the monomer mass if it survives electrophoresis. |
| Isoform 1 | Its mass relative to isoform 2 is not supplied. |
| Isoform 2 | Its mass relative to isoform 1 is not supplied. |
| Situation | Likely cause | Next action |
|---|---|---|
| Band higher than expected | The annotated homodimer may persist during electrophoresis. | Compare more strongly denaturing sample preparation and confirm band identity. |
| Band lower than expected | An isoform or unrelated protein could migrate below the predicted monomer; isoform masses are unknown. | Check with a second antibody or SULT1A1 depletion. |
| Multiple bands | Isoforms 1 and 2 may migrate differently, or some homodimer may persist. | Compare denaturing conditions and verify bands with SULT1A1 depletion. |
| Weak or no signal | SULT1A1 may be below detection in the tested lysate. | Check loading and antibody performance using a SULT1A1-positive lysate. |
| Fragments below expected size | Sample degradation or unrelated antibody binding may produce smaller bands. | Prepare fresh lysate with protease inhibitors and confirm bands with SULT1A1 depletion. |
Experiment evidence
Western blot images showing SULT1A1 band
A03500-1 · SULT1A1
A03500 · SULT1A1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for SULT1A1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for SULT1A1 in Western blot, you can use small intestine tissue lysate.
Positive control: Small intestine (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: Cytoplasmic localization supports lysate controls, but confirm the HPA not-detected adipose result by Western blot.
HPA tissue expression evidence for SULT1A1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Small intestine | enterocytes | High | Protein (IHC) | HPA → |
| Appendix | endocrine cells | Medium | Protein (IHC) | HPA → |
| Colon | enterocytes - Microvilli | Medium | Protein (IHC) | HPA → |
| Duodenum | glandular cells | Medium | Protein (IHC) | HPA → |
| Liver | hepatocytes | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Breast | adipocytes | Not detected | Protein (IHC) | HPA → |
| Bronchus | respiratory epithelial cells | Not detected | Protein (IHC) | HPA → |
| Caudate | glial cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced SULT1A1 Western Blot Tips
Deeper troubleshooting and optimisation questions for SULT1A1, answered from its protein features.
How should SULT1A1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could SULT1A1 isoforms produce different bands?
Isoforms · UniProt lists isoforms 1 and 2. In isoform 2, an alternative sequence replaces canonical residues 1–124, so the isoforms could differ in size or antibody recognition. Check whether the antibody targets the replaced region before assigning bands.
What phosphorylation should I consider when interpreting SULT1A1 bands?
PTM · UniProt annotates phosphoserine at position 138. This is a UniProt coordinate; paper or antibody numbering may differ. The annotation does not establish that phosphorylation produces a resolvable band shift.
Does this guide establish induction of SULT1A1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for SULT1A1 Western blot?
Transfer · Choose and verify transfer conditions for a protein near the predicted 34.2 kDa. Check membrane staining or a transfer control before interpreting a weak band; the supplied features do not specify a membrane or transfer method.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A03500-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should I quantify SULT1A1 across samples?
Quantitation · Quantify a consistently defined band near the expected 34.2 kDa and normalize to an appropriate loading measure. Because UniProt lists two isoforms, establish whether the antibody detects one or both before combining band intensities.
Why might SULT1A1 migrate differently from its predicted mass?
Interpretation · The predicted mass is 34.2 kDa, but no observed band position is supplied. Phosphorylation and alternative splicing are annotated; their presence alone does not establish a visible shift or explain any measured mass difference.
SULT1A1 is annotated as a homodimer, making an approximately doubled apparent mass worth investigating. The annotation does not show that a dimer survives Western blot preparation. Compare sample preparation conditions and antibody specificity before assigning the band.
SULT1A1 is annotated as a homodimer, making an approximately doubled apparent mass worth investigating. The annotation does not show that a dimer survives Western blot preparation. Compare sample preparation conditions and antibody specificity before assigning the band.
Boster reagents
SULT1A1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-SULT1A1 Antibody (C-term)
Cat # A03500-1
Real WB data
Anti-Sulfotransferase 1A1 SULT1A1 Antibody
Cat # A03500
Two the supplier anti-SULT1A1 antibodies have supplied Western blot images: A03500-1 with HepG2 and human lung lysates, and A03500 with mouse and rat liver lysates. These images document the tested samples, not performance across all tissues.
Which to pick: For human HepG2 or lung samples, A03500-1 has directly relevant WB images. For mouse or rat liver, choose A03500. A03500 also lists human reactivity, but its supplied WB image shows only mouse and rat liver.
Source: BosterBio SULT1A1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P50225.
- Human Protein Atlas. SULT1A1 tissue expression.
- PMC4765207 — target-verified WB comparison
- PMC4381599 — target-verified WB comparison
- PMC12849426 — target-verified WB comparison