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Product Info Summary
| SKU: | A03500-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-SULT1A1 Antibody (C-term)
View all Cytosolic Sulfotransferase 1A1/SULT1A1 Antibodies
SKU/Catalog Number
A03500-1
Size
80 µl
Description
Boster Bio Anti-SULT1A1 Antibody (C-term) (Catalog # A03500-1). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-SULT1A1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03500-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This SULT1A1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 246-279 amino acids of human SULT1A1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03500-1 is reactive to SULT1A1 in Human
Reconstitution
Calculated molecular weight
34165 Da
Background of Cytosolic Sulfotransferase 1A1/SULT1A1
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Has also estrogen sulfotransferase activity. responsible for the sulfonation and activation of minoxidil. Is Mediates the metabolic activation of carcinogenic N- hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03500-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
FC: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysates from HepG2 cell line and human lung tissue (from left to right), using SULT1A1 Antibody (C-term). A03500-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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Overlay histogram showing HepG2 cells stained with A03500-1 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A03500-1, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For SULT1A1 (Source: Uniprot.org, NCBI)
Gene Name
SULT1A1
Full Name
Sulfotransferase 1A1
Weight
34165 Da
Superfamily
sulfotransferase 1 family
Alternative Names
Sulfotransferase 1A1, ST1A1, Aryl sulfotransferase 1, HAST1/HAST2, Phenol sulfotransferase 1, Phenol-sulfating phenol sulfotransferase 1, P-PST 1, ST1A3, Thermostable phenol sulfotransferase, Ts-PST, SULT1A1, STP, STP1 SULT1A1 HAST1/HAST2, P-PST, PST, ST1A1, ST1A3, STP, STP1, TSPST1 sulfotransferase family 1A member 1 sulfotransferase 1A1|P-PST 1|aryl sulfotransferase 1|phenol-sulfating phenol sulfotransferase 1|sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1|thermostable phenol sulfotransferase1|ts-PST
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SULT1A1, check out the SULT1A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SULT1A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SULT1A1 Antibody (C-term) (A03500-1)
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