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- Table of Contents
SYCP1 / Synaptonemal complex protein 1 · Western blot design guide
Design a Western Blot for SYCP1
Source-linked SYCP1 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-SYCP1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
SYCP1 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~114.2 kDa | |
| Observed band | ~120 kDa | |
| Gel | 8% (catalog A06079-1) | |
| Positive control | Testis (IHC candidate; verify WB) | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | Testis positive control | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked SYCP1 Western Blot Protocol Options
The A06079-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human THP-1, rat testis, rat C6 (catalog A06079-1) |
| Gel % | 8% (catalog A06079-1) |
| Load | 30 ug; reducing conditions (catalog A06079-1) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A06079-1) |
| Membrane | nitrocellulose membrane (catalog A06079-1) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A06079-1) |
| Primary antibody | A06079-1 · 0.5 μg/mL (catalog A06079-1) |
| Primary incubation | overnight at 4°C (catalog A06079-1) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A06079-1) |
| Secondary incubation | 1.5 hour at RT (catalog A06079-1) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A06079-1) |
| Detection | ECL (catalog A06079-1) |
Section 2
What Is the Expected SYCP1 Western Blot Band Size?
SYCP1 is predicted at 114.2 kDa and observed near 120 kDa; the cause of the difference is not established.
| Band near 120 kDa | Matches the empirical SYCP1 band; confirm identity with appropriate controls |
| Band near 114 kDa | Near the predicted mass of the 976-residue SYCP1 chain |
| Band in testis lysate | Consistent with SYCP1's role in meiotic chromosome complexes |
| Weak or absent band in a whole-cell lysate | May reflect limited SYCP1 abundance outside meiotic cells |
💡Expected SYCP1 appearanceSYCP1 has a predicted mass of 114.2 kDa and an empirical band near 120 kDa; the cause of the difference is not established, so confirm band identity with appropriate controls.
| Predicted molecular mass | Sets the full-chain reference at 114.2 kDa |
| 976-residue sequence | Defines the predicted full-length chain without establishing its apparent migration |
| Homotetramer assembly | Could contribute to a higher band if assembly survives sample preparation; this is unverified |
| Coiled-coil structure | Might affect apparent migration; no shift is established by the supplied evidence |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | The sample may contain few cells undergoing meiosis | Check testis lysate as a positive sample and verify protein loading |
| Band higher than expected | Incomplete disruption of SYCP1 assemblies is possible | Review denaturation conditions and compare with the empirical band near 120 kDa |
| Band lower than expected | Protein breakdown or nonspecific antibody binding is possible | Check sample handling and confirm the band with an independent antibody or depletion control |
| Multiple bands | Breakdown products or nonspecific binding are possible | Compare bands with the 120 kDa reference using an independent antibody or depletion control |
| Weak or no signal | SYCP1 may be scarce outside meiotic chromosome complexes | Use a testis positive sample and check extraction, loading, and transfer |
| Fragments below expected size | SYCP1 may have degraded during preparation | Process samples promptly with protease inhibitors and confirm fragment identity |
Experiment evidence
Western blot images showing SYCP1 band
A06079-1 · SYCP1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for SYCP1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for SYCP1 in Western blot, you can use testis lysate, where HPA reports medium expression.
Positive control: Testis (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: Because SYCP1 is nuclear and chromosomal, use a lysate that retains nuclear proteins.
HPA tissue expression evidence for SYCP1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Testis | spermatogonia cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Appendix | glandular cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Breast | adipocytes | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced SYCP1 Western Blot Tips
Deeper troubleshooting and optimisation questions for SYCP1, answered from its protein features.
How should SYCP1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could a second SYCP1 band be an annotated isoform?
Isoforms · Only one isoform is supplied, with no alternative sequence listed. A second band cannot be assigned to an annotated SYCP1 isoform from these features alone.
Do annotated modifications explain a SYCP1 band shift?
PTM · No modified residues or glycosylation sites are listed. The supplied features therefore do not identify a modification that explains a shift, but they also do not establish why any particular band migrates differently.
Does this guide establish induction of SYCP1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for SYCP1?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A06079-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should the expected SYCP1 band be quantified?
Quantitation · Quantify the band near the reported 120 kDa position in comparable meiotic samples. Keep sample preparation consistent, since SYCP1 is associated with nuclear chromosomes and synaptonemal complexes.
Why does SYCP1 appear near 120 kDa instead of 114.2 kDa?
Interpretation · The predicted mass is 114.2 kDa, while the reported apparent band is about 120 kDa. The supplied features do not establish the cause of that difference; use the observed position as a guide when identifying the band.
SYCP1 is a meiotic synaptonemal complex component associated with nuclear chromosomes. Choose samples containing meiotic cells and account for its nuclear and chromosomal location when preparing lysates.
SYCP1 is described as a homotetramer with coiled coils, so oligomerization is relevant when assessing a higher band. These features alone do not show that an oligomer persists during Western blotting or identify the band.
The supplied record lists one 976-residue isoform and no propeptide or signal peptide. It does not support assigning a lower band to an annotated isoform or peptide cleavage product. Confirm band identity before interpreting it as SYCP1.
SYCP1 is a meiotic synaptonemal complex component associated with nuclear chromosomes. Choose samples containing meiotic cells and account for its nuclear and chromosomal location when preparing lysates.
SYCP1 is described as a homotetramer with coiled coils, so oligomerization is relevant when assessing a higher band. These features alone do not show that an oligomer persists during Western blotting or identify the band.
The supplied record lists one 976-residue isoform and no propeptide or signal peptide. It does not support assigning a lower band to an annotated isoform or peptide cleavage product. Confirm band identity before interpreting it as SYCP1.
Boster reagents
SYCP1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-SYCP1 Antibody Picoband®
Cat # A06079-1
A06079-1 is listed for human, mouse, and rat SYCP1. Its Western blot image shows an approximately 120 kDa band (expected 114 kDa) across the human cell, rat cell and testis, and mouse cell and testis lysates named in the caption. Evidence is limited to this product blot.
Which to pick: A06079-1 is the only listed option and has a Western blot image. Check its reported samples and conditions against your experiment; the pictured lysates do not establish performance in every human, mouse, or rat sample.
Source: BosterBio SYCP1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q15431.
- Human Protein Atlas. SYCP1 tissue expression.
- PMC12623929 — target-verified WB comparison
- PMC4751484 — target-verified WB comparison
- PMC2664383 — target-verified WB comparison