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Product Info Summary
| SKU: | A06079-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SYCP1 Antibody Picoband®
SKU/Catalog Number
A06079-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SYCP1 Antibody Picoband® catalog # A06079-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SYCP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06079-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human SYCP1 recombinant protein (Position: D95-H717). Human SYCP1 shares 75.8% and 76.6% amino acid (aa) sequence identity with mouse and rat SYCP1, respectively.
Reactive Species
A06079-1 is reactive to SYCP1 in Human, Mouse, Rat
Observed Molecular Weight
120 kDa
Calculated molecular weight
114.2 kDa
Background of SYCP1
Enables double-stranded DNA binding activity. Involved in protein homotetramerization. Predicted to be located in synaptonemal complex. Predicted to be active in central element; male germ cell nucleus; and transverse filament.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06079-1 is guaranteed for ELISA, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Human, Mouse, Rat |
| ELISA | 0.1-0.5 μg/ml |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of SYCP1 using anti-SYCP1 antibody (A06079-1).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: rat testis tissue lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse testis tissue lysates,
Lane 6: mouse TM4 whole cell lysates,
Lane 7: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYCP1 antigen affinity purified polyclonal antibody (A06079-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SYCP1 at approximately 120 kDa. The expected band size for SYCP1 is at 114 kDa.
Specific Publications For Anti-SYCP1 Antibody Picoband® (A06079-1)
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