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Designing an IHC experiment for TIMM8A? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the TIMM8A IHC GuideFacts about Mitochondrial import inner membrane translocase subunit Tim8 A.
Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and direct them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins like TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the overriding TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins.
| Human | |
|---|---|
| Gene Name: | TIMM8A |
| Uniprot: | O60220 |
| Entrez: | 1678 |

| Belongs to: |
|---|
| small Tim family |

DDP1deafness/dystonia peptide; DDPMGC12262; Deafness dystonia protein 1; DFN1; mitochondrial import inner membrane translocase subunit Tim8 A; MTSTIM8; TIM8A; translocase of inner mitochondrial membrane 8 (yeast) homolog A; translocase of inner mitochondrial membrane 8 homolog A (yeast); X-linked deafness dystonia protein
Mass (kDA):
10.998 kDA

| Human | |
|---|---|
| Location: | Xq22.1 |
| Sequence: | X; NC_000023.11 (101345661..101348742, complement) |
Highly expressed in fetal and adult brain, followed by fetal lung, liver and kidney. Also expressed in heart, placenta, lung, liver, kidney, pancreas, skeletal muscle and heart.
Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side.






PMID: 8841189 by Jin H., et al. A novel X-linked gene, DDP, shows mutations in families with deafness (DFN-1), dystonia, mental deficiency and blindness.
PMID: 10051608 by Koehler C.M., et al. Human deafness dystonia syndrome is a mitochondrial disease.