TRPM7 Antibodies

Transient receptor potential cation channel subfamily M member 7 (TRPM7)

Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death.

Involved in TNF- induced necroptosis downstream of MLKL by mediating calcium influx. The kinase activity is essential for the channel function.

Gene Information

Human
Gene Name:	TRPM7
Uniprot:	Q96QT4
Entrez:	54822

Family

Belongs to:
No superfamily

Alternative Names

CHAK; CHAK1FLJ25718; Channel-kinase 1; EC 2.7.11.1; Long transient receptor potential channel 7; LTRPC ion channel family member 7; LTrpC7; LTrpC-7; LTRPC7FLJ20117; transient receptor potential cation channel subfamily M member 7; transient receptor potential cation channel, subfamily M, member 7; transient receptor potential-phospholipase C-interacting kinase; TRP-PLIK

Molecular Weight

Mass (kDA):
212.697 kDA

Genomic Context

Human
Location:	15q21.2
Sequence:	15; NC_000015.10 (50557158..50686797, complement)

Subcellular Localizations

Membrane; Multi-pass membrane protein.

Diseases

• Malignant Neoplasms
• Ischemia
• Neoplasms
• Hypertensive Disease
• Brain Ischemia
• Magnesium Deficiency
• Tissue Adhesions
• Hypocalcemia
• Cerebrovascular Accident
• Adenocarcinoma
• Diabetes Mellitus
• Cardiovascular Diseases

• Malignant Neoplasm Of Breast
• Fibrosis
• Basophilic Leukemia
• Cerebral Ischemia
• Inflammation
• Leukemia
• Brain Injuries

Pathways

• Transport
• Cell Death
• Cell Proliferation
• Cell Growth
• Cell Adhesion
• Cell Migration
• Pathogenesis
• Excretion
• Localization
• Rna Interference
• Cell Cycle
• Intestinal Absorption
• Secretion

• Neuroprotection
• Immune Response
• Membrane Depolarization
• Cell Division
• Cell Differentiation
• Gene Silencing
• Reverse Transcription

PTMs

• Phosphorylation
• Cleavage

• Nitration
• Nitrosylation

• Dephosphorylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TRPM7

Boster Bio - Best Uses Of The TRPM7 Marker

If you're looking for the best TRPM7 antibodies, you've come the right place. Boster's antibody have been tested on multiple platforms, with known positive or negative samples, to ensure their high affinity. Boster offers an additional perk to scientists all over the world: they award product credits to first reviewers. To learn more about Boster Bio and its TRPM7 antibodies, read on.

TRPM7-shRNA

The TRPM7-shRNA marker is a pharmacologically tractable molecular target. It could be used as a therapeutic target in autoimmune diseases. Its role in immune cells, mast cells, and inflammatory response to immunoreactive substances has been demonstrated. But what are the best uses of it? Let's find out. Here are three uses for TRPM7'shRNA markers.

TRPM7 is a member the TRPM subfamily. This family includes all major ion channels found in the body. They are found in the brain, heart, and many other tissues. TRPM7 is essential for cell survival and proliferation of many kinds of cells. The TRPM7-shRNA marker was designed to detect this gene. The gene is found in different tissues including brain, heart, kidney, and other organs.

TNFa stimulated ASMCs. TNFa-induced changes in ASMCs were partially blocked by TRPM7, a marker that blocks TNF-a. The expression of TRPM7 -shRNA was also higher in rats who had been exposed to smoking than in normal mice. These findings indicate that the TRPM7-shRNA marker could be used to detect the effects of tobacco smoke.

Guangzhou Forevergen Co., Ltd. conceived the TRPM7–shRNA. The sequence of TRPM7-shRNA is 5'-GATCCCCGTCCGTCGGTCACAGCTCTGGAAGAACGGG-3'. Its counterpart, scramble shRNA, has scrambled sequencing. These data can help determine the effects on osteogenesis of two different treatments.

TRPM7 is involved in osteogenesis as well as calcium and magnesium homeostasis. Its absence in skeletal tissue has been linked to defects in skeletogenesis, delayed intramembranous ossification, and accelerated endochondral ossification in zebrafish.

It is also known TRPM7 interacts F-actin, which regulates microfilament assembly dynamics. Although it may act as a mechanical mediator in some cases, the exact mechanism is not known. Researchers will be able to study the relationship between TRPM7 RNA and actin filament dynamics in different cell types using the TRPM7-shRNA marker. It is, however one of the most promising tools in studying actin dynamics.

It has multiple roles in immune cells, including regulating inflammatory signal transduction and proliferation in macrophages. It is therefore important to understand how TRPM7 affects your nervous system. T1DM can also be affected if TRPM7 is not present in the blood brain barrier or at the blood glucose level. T1DM is associated with damage to the nervous system, and silencing TRPM7 may impair spatial cognitive function by inhibiting the proliferation of hippocampal nerves.

RTPCR for TRPM7

RTPCR for TRPM7 genes was developed to detect knockdown in human VSMCs. TRPM7 is a gene that regulates Ca2+ homeostasis. Transfected siRNAs into mouse VSMCs to knock down TRPM7 resulted in a significant decrease in protein levels. This marker is also present within human VSMCs.

The expression levels of TRPM7 genes can be determined by RT-PCR using immunofluorescence or western blotting. RT-PCR can also use whole-cell patch-clamp methods to measure TRPM7 electrons. MTT assays were employed to measure cell viability, proliferation, and transwell experiments were used in order to evaluate cell migration and invasion. Other proteins were measured by Western blotting and patch clamp recordings.

The RT-PCR for the TRPM7 gene can detect both exon and nasopharyngeal cancer cells. It regulates calcium signaling and cell movement. TRPM7 decreases cell migration and invasion. Moreover, silencing of the gene reduces cell viability. This means that the gene may be functional in the treatment and prevention of cancer. Further research is needed to understand the mechanisms controlling the function TRPM7 gene.

TRPM7 was significantly reduced in expression by RNAi silencing. Similar results were obtained for t-Akt, p-ERK1/2 and TRPM7 silencing. The silencing TRPM7 gene didn't significantly alter the expression of tAkt or pERK1/2, but it did decrease the expression MMP-2.

Transient receiver potential melastatin7, also known as TRPM7, is a TRP-channel family member. It plays many physiological roles. The regulation of magnesium absorption is also regulated by the TRPM7 gene. Its homolog TRPM6, which is a close relative, has been implicated with the regulation of Mg 2+ absorption in the distal convoluted tubeule of kidney.

In this study, we employed a genetic perturbation model to examine the role of TRPM7 in human glioblastoma cells. Cell viability was evaluated by sulforhodamine B staining. To determine the role of TRPM7 in these processes, cell migration, invasion, colony and tumorsphere formation were all performed. TRPM7 could be a promising therapeutic target for the treatment glioblastoma.

ICC to TRPM7

Researchers recently discovered that TRPM7 channels participate in the pacemaking activity ICCs. TRPM7, a mammalian TRP homologue, is required for murine intestinal ratemaking. This discovery may be the foundation for a new treatment for gastrointestinal motility disorder. We present here our data on the pharmacological characteristics of TRPM7 in ICCs. This information will assist future researchers in developing drugs that target TRPM7.

TRPM7 regulates vesicle fission during endocytosis. The short-term synaptic depressibility of neurons with KO to TRPM7 is characterized by a slower kinetics, increased number and slower rate of endocytic activity. This suggests that TRPM7 may be able to control the number of neuronal endocytic vessels by controlling the kinetics single endocytic events.

Previous whole-cell recordings from chromaffin-cell chromaffin-cell cells were conducted at a -80mV resting membrane potential. This may have resulted in TRPM7 currents being missed due to the Mg2+ intracellular solution that substantially inhibits TRPM7 activation. This has been corrected in our revised manuscript. The authors will discuss this in detail in the discussion. We are currently developing a protocol to measure TRPM7 within human neuronal cells.

To detect TRPM7 in frozen brain sections of rats, we first used an intracellular antibody. We used this antibody in a concentration of 1:600, followed by goat anti-rabbit-AlexaFluor-488 staining. Neurons showed green immunoreactivity. Further, we used a blocking protease that blocked TRPM7 to stop the antibody staining cell nuclei.

Melastatin is a voltage-independent Ca2+ permeability cation channel that is expressed in mammalian cellular cells. They play central roles in Mg2+ ion homeostasis and regulate anoxic neuronal cell death. They also play a role in the regulation, according to metabolic state, of calcium and magnesium Ion Fluxes in Plasma Membrane.

Immunofluorescence to TRPM7

For TRPM7 immunohistochemistry, antibody LS-C230786 (aa 1817-1863) is an RPE-conjugated monoclonal antibody that recognizes human, mouse, and rat proteins. It can be used in ICC, IF and IHC. The antibody is shipped in vials with trehalose and sodium chloride.

When cells were incubated in 2-APB or at pH 5.0, the fluorescence levels of TRPM6- and TRPM7 decreased. The fluorescence of cardiac myocytes decreased by 2.APB from 0.1748 to 0.0383 a.u. To $0.03833 a.u. (n = 6-64). This result is consistent with previous observations indicating that TRPM6 fluorescence was unaffected by extracellular acidification.

ARL15 interacts with TRPM7 when CNNM3 has been present but does not suppress TRPM7 Currents when CNNM3 has been absent. The authors should explain the significance of this effect, because the ARL15 does not interact with TRPM7 physically. Additionally, all three CNNM proteins are present in all cell lines. They should discuss the importance and interplay between TRPM7, CNNM, and cell signaling.

MyBioSource, Inc. purchased the antibodies used for detecting TRPM6 in human tissue homogenates and TRPM7. The antibody was used together with the ELISA kit. The sample and standard were prepared in equal volumes and one well served as a blank. The samples and standard were then added to the microtiter plates, and a biotin-conjugated antibody preparation was used for immunohistochemistry. The signal was quantified using an ImageJ software program.

TRPM7 is a plasma membrane cation channel that contains a functional kinase in the C terminus. Researchers used this approach to identify interactions between TRPM7, ARL15, and found that increasing levels ARL15 decreased TRPM7 currents within Xenopus oocytes. They concluded that TRPM7 plays a role in the regulation of Mg2+ homeostasis, pace-making, and pace-making.

The protein co-expresses in cardiomyocytes belonging to all four chambers. In vitro studies have shown that TRPM6 as well as TRPM7 are both co-expressed within the same cells. Researchers must determine how they communicate in order to understand their roles. The same mechanism is responsible to the TRPM6 inhibitory cell currents in heart. It is therefore crucial to find the right drugs that inhibit TRPM6 in the body.