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Product Info Summary
| SKU: | M00789 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-TRPM7 Monoclonal Antibody
SKU/Catalog Number
M00789
BM5443 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TRPM7 Monoclonal Antibody catalog # M00789. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRPM7 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00789)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADGD-20
Isotype
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Immunogen
A synthesized peptide derived from human TRPM7
Reactive Species
M00789 is reactive to TRPM7 in Human, Mouse, Rat
Observed Molecular Weight
213 kDa
Calculated molecular weight
212.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00789 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
FC 1:100
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of TRPM7 expression in HeLa cell lysate.
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LBQ657 significantly extenuated TGF-β1 induced cardiac fibroblast activation in MEF. (A) mRNA expression of Mmp9 and α -Sma in control MEF, MEF treated with TGF-β1 and MEF treated with LBQ657 + TGF-β1. (B) Protein levels of MMP9 and α-SMA in the rats. (C) The efficiency of the siRNA used to reduce the mRNA expression of TRPM7 in MEF. (D) mRNA expression of Trpm7 , Mmp9 and α -Sma in MEF treated with TGF-β1, MEF transfected with si-TRPM7 and treated with TGF-β1, MEF treated with LBQ657 + TGF-β1. (E) Protein levels of TRPM7, MMP9 and α-SMA in the rats. The data presented are mean ± SD. Each group contained the results of three independent repeated trials. 18S RNA was used as the internal reference gene and β-actin was used as the internal reference protein for normalization and statistical analysis. ∗∗∗∗ P < 0.0001, ∗∗∗ P < 0.001, and ∗∗ P < 0.01.
Index in PubMed under a CC BY license. PMID: 34778271
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LBQ657 protected against fibrosis by blocking TRPM7 channel. (A) The virtual binding mode of NEPi and natural cholesterol hemisuccinateon ligand (as control) on TRPM7. NEPi (red) and cholesterol hemisuccinate (gray) are shown as a stick model. (B) Current amplitude of TRPM7-like current recorded in neonatal rat cardiac fibroblasts in control cell (black), LBQ657-10 μM treated cell (blue) and LBQ657-50 μM treated cell (red). (C) mRNA expression of Mmp9 and α -Sma in MEF treated with TGF-β1, MEF treated with 2-APB + TGF-β1, and MEF treated with LBQ657 + TGF-β1. (D) Protein levels of MMP9 and α-SMA in the rats. The data presented are mean ± SD. Each group contained the results of three independent repeated trials. 18S RNA was used as the internal reference gene and β-actin was used as the internal reference protein for normalization and statistical analysis. ∗∗∗∗ P < 0.0001, ∗∗∗ P < 0.001, and ∗∗ P < 0.01.
Index in PubMed under a CC BY license. PMID: 34778271
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LBQ657 reduced hypoxia-induced necrosis by inhibiting TRPM7-mediated Ca 2+ influx in cardiomyocytes. (A) Comparison of TRPM7 protein levels in MEF and H 9 C 2 cells. (B) Fluorescence intensity of Fluo-4-AM fluorescent dye in 488 nm excitation light of normoxic H 9 C 2 , hypoxic H 9 C 2 and hypoxic H 9 C 2 pre-treated with LBQ657. (C) Protein levels of caspase3, cleaved caspase3, LC3B-I and LC3B-II in hypoxic H 9 C 2 and hypoxic H 9 C 2 pre-treated with LBQ657. (D) Protein levels of RIPK1/RIPK3/MLKL/p-MLKL in hypoxic H 9 C 2 and hypoxic H 9 C 2 pre-treated with LBQ657. (E) Protein levels of RIPK1/RIPK3/MLKL/p-MLKL in normoxic H 9 C 2 and normoxic H 9 C 2 treated with LBQ657. (F) Protein levels of RIPK1/RIPK3/MLKL/p-MLKL in hypoxic H 9 C 2 and hypoxic H 9 C 2 pre-treated with BAPTA-AM. The data presented are mean ± SD. Each group contained the results of three independent repeated trials. β-actin was used as the internal reference protein for normalization and statistical analysis. ∗∗∗∗ P < 0.0001, ∗∗∗ P < 0.001, ∗∗ P < 0.01, and ∗ P < 0.05.
Index in PubMed under a CC BY license. PMID: 34778271
Specific Publications For Anti-TRPM7 Monoclonal Antibody (M00789)
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Customer Q&As
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6 Customer Q&As for Anti-TRPM7 Monoclonal Antibody
Question
Is a blocking peptide available for product anti-TRPM7 Monoclonal antibody (M00789)?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
We do provide the blocking peptide for product anti-TRPM7 Monoclonal antibody (M00789). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-07
Question
Will anti-TRPM7 Monoclonal antibody M00789 work on horse WB with colon placenta?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
Our lab technicians have not tested anti-TRPM7 Monoclonal antibody M00789 on horse. You can run a BLAST between horse and the immunogen sequence of anti-TRPM7 Monoclonal antibody M00789 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse colon placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-30
Question
Will anti-TRPM7 Monoclonal antibody M00789 work for IF with liver?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
According to the expression profile of liver, TRPM7 is highly expressed in liver. So, it is likely that anti-TRPM7 Monoclonal antibody M00789 will work for IF with liver.
Boster Scientific Support
Answered: 2019-08-20
Question
I was wanting to use your anti-TRPM7 Monoclonal antibody for IF for rat liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat liver identification?
Verified Customer
Verified customer
Asked: 2017-09-19
Answer
As indicated on the product datasheet, M00789 anti-TRPM7 Monoclonal antibody has been validated for Flow Cytometry, IF, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-19
Question
I have a question about product M00789, anti-TRPM7 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Mitchell
Verified customer
Asked: 2016-06-24
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00789 anti-TRPM7 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-06-24
Question
Is this M00789 anti-TRPM7 Monoclonal antibody reactive to the isotypes of TRPM7?
S. Brown
Verified customer
Asked: 2013-10-28
Answer
The immunogen of M00789 anti-TRPM7 Monoclonal antibody is A synthesized peptide derived from human TRPM7. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-10-28
