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- Table of Contents
UVRAG / UV radiation resistance-associated gene protein · Western blot design guide
Design a Western Blot for UVRAG
Real validated UVRAG Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-UVRAG WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
UVRAG Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~78.2 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Fallopian tube (IHC candidate; verify WB) +3 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated | |
| Caveat | Phosphorylation-state controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 2 isoform(s) |
Section 1
Real Curated UVRAG Western Blot Protocols
The A02385 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | mouse lung tissue lysate (catalog A02385) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A02385 · 1 μg/ml (catalog A02385) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected UVRAG Western Blot Band Size?
UVRAG is predicted at 78.2 kDa; isoforms and phosphorylation could affect migration, but no empirical band size or visible shift is established.
| Single band near 78.2 kDa | Consistent with the predicted UVRAG mass; confirm identity with an appropriate control. |
| Two bands at different positions | Could reflect isoforms 1 and 2; their migration difference is not established. |
| Closely spaced doublet | Could reflect different phosphorylation states; a visible shift is not established. |
| Band away from 78.2 kDa | Could represent an isoform or another species; confirm UVRAG identity. |
💡Expected UVRAG appearanceUVRAG has a predicted mass of 78.2 kDa, but no empirical band size is supplied; isoforms and phosphorylation could affect migration, so confirm band identity with appropriate controls.
| Predicted UVRAG mass | Places the reference band near 78.2 kDa. |
| Isoforms 1 and 2 | May differ in size, but their individual masses and migration are not supplied. |
| Phosphoserine at residue 498 by MTOR | May affect migration; no visible shift is established. |
| Phosphothreonine at residue 518 | May affect migration; no visible shift is established. |
| Situation | Likely cause | Next action |
|---|---|---|
| Band higher than expected | An isoform or phosphorylation state could affect migration; the cause is unconfirmed. | Compare with a UVRAG knockdown control and assess phosphorylation if relevant. |
| Band lower than expected | An isoform or UVRAG fragment is possible; its size is not established. | Check antibody epitope coverage and compare with a UVRAG knockdown control. |
| Multiple bands | Isoforms 1 and 2 or different phosphorylation states are possible. | Identify UVRAG-dependent bands with a knockdown control; assess phosphorylation if relevant. |
| Weak or no signal | UVRAG recovery or antibody detection may be insufficient. | Check lysate loading and antibody performance with a positive control. |
| Fragments below expected size | Protein degradation during sample preparation is possible. | Prepare fresh lysate with protease inhibitors and compare antibody epitopes. |
Experiment evidence
Western blot images showing UVRAG band
A02385 · UVRAG
A02385-2 · UVRAG
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for UVRAG Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for UVRAG in Western blot, you can use fallopian tube lysate, which shows medium HPA staining.
Positive control: Fallopian tube (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: UVRAG is intracellular, but HPA reports only medium staining in positive tissues, so signal may be modest.
HPA tissue expression evidence for UVRAG
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Fallopian tube | glandular cells | Medium | Protein (IHC) | HPA → |
| Hippocampus | glial cells | Medium | Protein (IHC) | HPA → |
| Lung | alveolar cells | Medium | Protein (IHC) | HPA → |
| Thyroid gland | glandular cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Appendix | glandular cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Breast | adipocytes | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced UVRAG Western Blot Tips
Deeper troubleshooting and optimisation questions for UVRAG, answered from its protein features.
How should UVRAG band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
How could UVRAG isoforms affect band interpretation?
Isoforms · UniProt lists isoforms 1 and 2. Isoform 2 lacks residues 1–372 of the canonical sequence and could produce a smaller band. Its apparent mass is not supplied; verify the antibody’s isoform coverage before assigning bands.
Choose an epitope within the sequence retained by isoform 2, beyond canonical residue 372. An antibody against residues 1–372 would not be expected to detect isoform 2. Confirm the antibody’s stated epitope and isoform reactivity.
Choose an epitope within the sequence retained by isoform 2, beyond canonical residue 372. An antibody against residues 1–372 would not be expected to detect isoform 2. Confirm the antibody’s stated epitope and isoform reactivity.
Which UVRAG phosphorylation sites matter when comparing bands?
PTM · UniProt lists canonical phosphoserines at 493, 498, 508, 522, 549, 550, 571, 582 and 689, plus phosphothreonine at 518. Record these as UniProt canonical coordinates when choosing a phosphosite antibody. Their presence does not establish a resolvable band shift.
UniProt identifies phosphoserine 498 as modified by MTOR. A phospho-S498 assay can target that specific modification; a total UVRAG blot cannot establish its phosphorylation state. S498 is the UniProt canonical coordinate, which may differ from an antibody’s numbering convention.
UniProt identifies phosphoserine 498 as modified by MTOR. A phospho-S498 assay can target that specific modification; a total UVRAG blot cannot establish its phosphorylation state. S498 is the UniProt canonical coordinate, which may differ from an antibody’s numbering convention.
Does this guide establish induction of UVRAG?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for UVRAG?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A02385 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should UVRAG be quantified across samples?
Quantitation · Quantify the same defined band across samples and check that the antibody detects the intended isoform. UVRAG is listed at endosomes, lysosomes, autophagosomes and endoplasmic reticulum, so keep sample preparation consistent when comparing fractions or lysates.
Should UVRAG migrate at its predicted 78.2 kDa?
Interpretation · The canonical protein has a predicted mass of 78.2 kDa. No observed band position is supplied, so use this as a reference rather than an expected apparent mass. The listed modifications alone do not establish a visible shift.
Check whether a smaller band is compatible with isoform 2, which lacks canonical residues 1–372, and whether the antibody can recognize it. For bands near the canonical protein, compare with the 78.2 kDa predicted mass without assuming phosphorylation explains a difference. No empirical UVRAG band position is supplied.
Check whether a smaller band is compatible with isoform 2, which lacks canonical residues 1–372, and whether the antibody can recognize it. For bands near the canonical protein, compare with the 78.2 kDa predicted mass without assuming phosphorylation explains a difference. No empirical UVRAG band position is supplied.
Boster reagents
UVRAG Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-UVRAG Antibody
Cat # A02385
Real WB data
Anti-UVRAG Antibody
Cat # A02385-2
Two the supplier anti-UVRAG antibodies list WB images. A02385 shows mouse lung tissue lysate at 1 μg/ml; A02385-2 shows extracts from various cell lines. The latter caption does not identify the cell lines or their species.
Which to pick: For mouse lung under the reported conditions, choose A02385. Both list human and mouse reactivity; only A02385-2 also lists rat. Its WB image uses various cell lines without naming them, so the image alone does not establish rat WB performance.
Source: BosterBio UVRAG gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q9P2Y5.
- Human Protein Atlas. UVRAG tissue expression.
- PMC4826239 — target-verified WB comparison
- PMC10177159 — target-verified WB comparison
- PMC6613838 — target-verified WB comparison