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Product Info Summary
| SKU: | A02385 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-UVRAG Antibody
SKU/Catalog Number
A02385
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-UVRAG Antibody (Catalog # A02385). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
UVRAG antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-UVRAG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02385)
Host
Rabbit
Contents
UVRAG antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
UVRAG antibody was raised against an 18 amino acid peptide near the carboxy terminus of human UVRAG. The immunogen is located within amino acids 490 - 540 of UVRAG.
Cross-reactivity
UVRAG antibody is human and mouse reactive.
Reactive Species
A02385 is reactive to UVRAG in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
78.2 kDa
Background of UVRAG
The class III type phosphoinositide 3-kinase (PI3KC3) / Vps34 regulates vacuolar trafficking as well as autophagy (1). UVRAG (UV radiation resistance-associated gene) is associated with the Beclin-1/PI3KC3 complex and promotes PI3KC3 enzymatic activity and autophagy, while suppressing proliferation (2). UVRAG is highly expressed in brain, lung, kidney and liver and contains one C2 domain, which is involved in calcium-dependent phospholipid binding. Beclin-1 binding to UVRAG promotes both autophagosome maturation and endocytic trafficking (3). UVRAG is also a potential tumor suppressor protein with frameshift mutations observed in colon and gastric carcinomas (4,5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02385 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
UVRAG antibody can be used for detection of UVRAG by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of UVRAG in mouse lung tissue lysate with UVRAG antibody at 1 μg/ml.
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Immunohistochemistry of UVRAG in human lung tissue with UVRAG antibody at 5 μg/ml.
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Immunofluorescence of UVRAG in human lung tissue with UVRAG antibody at 20 μg/ml.
Specific Publications For Anti-UVRAG Antibody (A02385)
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