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Product Info Summary
| SKU: | AZK4IAH1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Zebrafish AGO4 Antibody Picoband®
SKU/Catalog Number
AZK4IAH1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish AGO4 Antibody Picoband® catalog # AZK4IAH1. Tested in IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Immunohistochemistry applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20 ℃ for one year from date of receipt. After reconstitution, at 4 ℃ for one month. It can also be aliquotted and stored frozen at -20 ℃ for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish AGO4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZK4IAH1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish AGO4 recombinant protein (Position: K116-L161).
Reactive Species
AZK4IAH1 is reactive to AGO4 in Zebrafish
Background of AGO4
AGO4 (Argonaute 4) is also known as EIF2C4. This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic containing PAZ and PIWI domains, and it may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and eukaryotic translation initiation factor 2C, 1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZK4IAH1 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Positive Control
IHC: zebrafish cerebellum tissue
Validation Images & Assay Conditions
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IHC analysis of Zebrafish AGO4 using anti-Zebrafish AGO4 antibody (AZK4IAH1).
Zebrafish AGO4 was detected in paraffin-embedded section of zebrafish cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Zebrafish AGO4 Antibody (AZK4IAH1) overnight at 4°C.Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish AGO4 Antibody Picoband® (AZK4IAH1)
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