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Product Info Summary
| SKU: | AZA4QNW7 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Zebrafish PHYHIPL/b Antibody
SKU/Catalog Number
AZA4QNW7
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish PHYHIPL/b Antibody catalog # AZA4QNW7. Tested in IHC applications. This antibody reacts with Zebrafish.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish PHYHIPL/b Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA4QNW7)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish PHYHIPL/b recombinant protein (Position: M1-D337).
Reactive Species
AZA4QNW7 is reactive to PHYHIPL/b in Zebrafish
Background of PHYHIPL/b
Phytanoyl-CoA hydroxylase-interacting protein-like is an enzyme that in humans is encoded by the PHYHIPL gene. PHYHIPL (phytanoyl-CoA 2-hydroxylase interacting protein-like), also known as phytanoyl-CoA hydroxylase-interacting protein-like, is a 376 amino acid protein that contains one fibronectin type-III domain and belongs to the PHYHIP family. Conserved in chimpanzee, canine, mouse, rat, chicken, zebrafish and Caenorhabditis elegans, PHYHIPL exists as three alternatively spliced isoforms. PHYHIPL is a down-regulated target of IRX1, a homeobox tumor suppressor gene linked to gastric carcinoma. PHYHIPL may also play a role in the development of the central system. The gene that encodes PHYHIPL maps to human chromosome 10q21.1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZA4QNW7 is guaranteed for IHC Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Immunohistochemistry (IHC) | 2-5 μg/ml | Zebrafish |
Validation Images & Assay Conditions
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IHC analysis of PHYHIPL/b using anti-PHYHIPL/b antibody (AZA4QNW7).
PHYHIPL/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PHYHIPL/b Antibody (AZA4QNW7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of PHYHIPL/b using anti-PHYHIPL/b antibody (AZA4QNW7).
PHYHIPL/b was detected in a paraffin-embedded section of zebrafish spinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PHYHIPL/b Antibody (AZA4QNW7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish PHYHIPL/b Antibody (AZA4QNW7)
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