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Product Info Summary
| SKU: | EK0382 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine. |
Product info
Product Name
Human IGFBP-1 ELISA Kit PicoKine®
SKU/Catalog Number
EK0382
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IGFBP-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human IGFBP1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IGFBP-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0382)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A26-N259
Sensitivity
<1 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0382 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0382 is reactive to IGFBP1 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Application Guarantee
EK0382 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IGFBP-1
IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-1, consisting of 234 amino acids with the molecular mass of 25KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0382-CAP | Anti-Human IGFBP1 Pre-coated 96-well strip microplate | 1 |
| EK0382-ST | Human IGFBP1 Standard | 2 vials, 10 ng/tube |
| EK0382-DA | Human IGFBP1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.042 | 0.075 | 0.140 | 0.225 | 0.386 | 0.701 | 1.134 | 1.941 |
Data Example Images
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Human IGFBP-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IGFBP-1, Dilution ratio of 1:20, concentration in serum and plasma is 159-75205 pg/ml..
Some articles we found to cite concentrations of IGFBP-1 in samples: 24273693 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IGFBP-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 66 | 243 | 1016 | 68 | 225 | 1048 |
| Standard deviation | 3.23 | 17.25 | 67.05 | 3.67 | 19.8 | 86.98 |
| CV (%) | 4.9% | 7.1% | 6.6% | 5.4% | 8.8% | 8.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IGFBP-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 66 | 74 | 68 | 68 | 69 | 3 | 4.3% |
| Sample 2 | 243 | 252 | 252 | 256 | 250 | 4.76 | 1.9% |
| Sample 3 | 1016 | 979 | 1037 | 933 | 991 | 39.52 | 3.9% |
Protein Target Info & Infographic
Gene/Protein Information For IGFBP1 (Source: Uniprot.Org, NCBI)
Gene Name
IGFBP1
Full Name
Insulin-like growth factor-binding protein 1
Weight
27904 MW
Alternative Names
Insulin-like growth factor-binding protein 1;IBP-1;IGF-binding protein 1;IGFBP-1;Placental protein 12;PP12;IGFBP1;IBP1; IGFBP1 AFBP, IBP1, IGF-BP25, PP12, hIGFBP-1 insulin like growth factor binding protein 1 insulin-like growth factor-binding protein 1|IBP-1|IGF-binding protein 1|IGFBP-1|alpha-pregnancy-associated endometrial globulin|amniotic fluid binding protein|binding protein-25|binding protein-26|binding protein-28|growth hormone independent-binding protein|placental protein 12
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IGFBP1, check out the IGFBP1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IGFBP1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IGFBP-1 ELISA Kit PicoKine® (EK0382)
Hello CJ!
EK0382 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Decreased expression of NR4A nuclear receptors in adenomyosis impairs endometrial decidualization
Krüppel-like factor 12 negatively regulates human endometrial stromal cell decidualization
Inflammation-Associated Cytokines IGFBP1 and RANTES Impair the Megakaryocytic Potential of HSCs in PT Patients after Allo-HSCT
Activation of Uterine Smad3 Pathway Is Crucial for Embryo Implantation
Krüppel-like factor 12 negatively regulates human endometrial stromal cell decidualization
Zhang S, Zhao Y, Hu X, Liu Z, Chen X, Chen X, Du J. Mol Cell Endocrinol. 2013 Aug 25;376(1-2):33-42. Doi: 10.1016/J.Mce.2013.05.025. Epub 2013 Jun 6. Distinct Post-Transcriptional Regulation Of Igfbp1 Gene By Hypoxia In Lowland Mouse And Qinghai-T...
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Human IGFBP-1 ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated IGFBP-1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IGFBP-1 antigen?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-03-23
Question
Hi support; I would like to ask if there is cross-reactivity with murine IGFBP-1 levels in the EK0382 Human IGFBP-1 ELISA Kit as I would like to know the total level of IGFBP-1.
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
The immunogen used in EK0382 Human IGFBP-1 ELISA Kit was a recombinant human IGFBP-1 (This is the full length protein minus the signal peptide). However, epitope mapping haven
Boster Scientific Support
Answered: 2020-02-13
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-08-19
Question
Q: Can IGFBP-1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-04-11
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-04-11
Question
Q: What is the optimal O.D. value for IGFBP-1 ELISA kit? I used your IGFBP-1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IGFBP-1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-03-28
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IGFBP-1 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of IGFBP-1 that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2019-03-28
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-02-23
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-02-23
Question
Q: can I use citrate plasma as samples in Human IGFBP-1 Picokine® ELISA Kit (Catalog # EK0382)?
D. Rodriguez
Verified customer
Asked: 2019-01-21
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of IGFBP-1 causing degradation of its protein structure. IGFBP-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IGFBP-1 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-01-21
Question
Q: which procedure should I follow in order to thaw whole blood sample for IGFBP-1 ELISA after freezing?
T. Bhatt
Verified customer
Asked: 2018-06-27
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IGFBP-1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-06-27
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-03-02
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-03-02
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2014-10-02
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2014-10-02
