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- Table of Contents
Egf antibody (A00378-1) performs well in IHC, WB, ELISA (Cap) and shows reactivity with Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 12 Publications
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IHC analysis of EGF using anti-EGF antibody (A00378-1).
EGF was detected in paraffin-embedded section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EGF Antibody (A00378-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
EGF was detected in paraffin-embedded section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EGF Antibody (A00378-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Anti-EGF Antibody Picoband®
Egf antibody
| Reactive Species | Host | Application |
|---|---|---|
| Rat | Rabbit | IHC, WB, ELISA (Cap) |
Tested Applications
Egf antibody was positively detected by IHC in:
rat kidney tissue, rat small intestine tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Rat
ELISA(Cap)
1-5μg/ml
Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-EGF Antibody Picoband®
View all EGF Antibodies
View all EGF Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat EGF recombinant protein (Position: N974-R1026). Rat EGF shares 69.8% and 77.4% amino acid (aa) sequence identity with human and mouse EGF, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
6 kDa
Calculated Molecular Weight
124.1 kDa
Background of Egf
EGF is known as epidermal growth factor. This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. Additionally, it acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Cite This Product
Anti-EGF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00378-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Rat
100%
High Homology
Mouse
77%
Moderate Homology
Rabbit
77%
Moderate Homology
Dog
72%
Moderate Homology
Monkey
72%
Moderate Homology
Human
70%
Moderate Homology
Zebrafish
53%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for Egf antibody
Publications
Application:
Species:
Submandibular Gland Pathogenesis Following SARS-CoV-2 Infection and Implications for Xerostomia
Published: 2024-06-21
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: IF
Impact Factor: 4.90
Species: Mouse
PMID: 38999930
Tissue-engineered edible bird’s nests (TeeBN)
Published: 2023-02-21
Journal: International Journal of Bioprinting
Application: Dot blot
Impact Factor: 8.40
PMID: 37457942
Venlafaxine increases aromatization, reduces apical V-ATPase in clear cells and induces increased number of mast cells and smooth muscle cells death in rat cauda epididymis
Published: 2022-12-28
Journal: LIFE SCIENCES
Application: IF
Impact Factor: 6.78
Species: Rat
PMID: 36584913
Egg yolk oil accelerates wound healing in streptozotocin induced diabetic rats
Published: 2022-08-30
Journal: BIOTECHNIC & HISTOCHEMISTRY
Application: IHC
Impact Factor: 1.83
Species: Rat
PMID: 36040350
Venlafaxine-induced adrenergic signaling stimulates Leydig cells steroidogenesis via Nur77 overexpression: A possible role of EGF
Published: 2021-10-21
Journal: LIFE SCIENCES
Application: WB,IF
Impact Factor: 5.04
Species: Rat
PMID: 34688693
Biomimetic hydrogel for rapid and scar-free healing of skin wounds inspired by the healing process of oral mucosa
Published: 2019-10-10
Journal: Acta Biomaterialia
Application: IHC
Impact Factor: 6.64
Species: Rat
PMID: 31606531
The Antimicrobial Peptide Nal-P-113 Exerts a Reparative Effect by Promoting Cell Proliferation, Migration, and Cell Cycle Progression
Published: 2018-09-12
Journal: Biomed Research International
Application: IHC
Impact Factor: 2.58
Species: Rat
PMID: 30298136
Therapeutic Effect and Mechanism of Oxytropis falcata Gel on Deep Second-Degree Burn in Rats
Published: 2017-11-13
Journal: Evidence-based Complementary and Alternative Medicine
Application: IHC
Impact Factor: 1.74
Species: Rat
PMID: 29259644
Proton pump inhibitors are not the key for therapying non-steroidal anti-inflammatory drugs-induced small intestinal injury
Published: 2013-04-19
Journal: RHEUMATOLOGY INTERNATIONAL
Application: IHC
Impact Factor: 2.21
Species: Rat
PMID: 23604681
Transdermal delivery of human epidermal growth factor facilitated by a peptide chaperon
Published: 2013-01-11
Journal: EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
Application: IF
Impact Factor: 3.50
Species: Rat
PMID: 23385091
Interactive actions of prostaglandin and epidermal growth factor to enhance proliferation of granulosa cells from chicken prehierarchical follicles
Published: 2007-02-14
Journal: PROSTAGLANDINS & OTHER LIPID MEDIATORS
Application: ICC
Impact Factor: 1.95
Species: Chicken
PMID: 17499748
Effect of IL-10 on the expression of HSC growth factors in hepatic fibrosis rat
Published: 2005-08-21
Journal: WORLD JOURNAL OF GASTROENTEROLOGY
Application: ICC
Impact Factor: 0.00
Species: Rat
PMID: 16097045
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Product Promise
We promise Anti-EGF Antibody Picoband® performs excellently in relevant applications for IHC, WB, ELISA (Cap). If it doesn't perform as promised, we will refund the product. Learn more
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A00378-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026