Anti-EGF Antibody Picoband™

Product Name

Anti-EGF Antibody Picoband™

View all EGF Antibodies

SKU/Catalog Number

A00378-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-EGF Antibody Picoband™ catalog # A00378-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EGF Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00378-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived rat EGF recombinant protein (Position: N974-R1026). Rat EGF shares 69.8% and 77.4% amino acid (aa) sequence identity with human and mouse EGF, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00378-1 is reactive to EGF in Rat

Applications

A00378-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

6 kDa

Calculated molecular weight

133.994kDa

Background of EGF

EGF is known as epidermal growth factor. This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. Additionally, it acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, By Heat
ELISA(Cap) , 1-5μg/ml, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of EGF using anti-EGF antibody (A00378-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant rat EGF protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGF antigen affinity purified polyclonal antibody (Catalog # A00378-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGF at approximately 6KD. The expected band size for EGF is at 6KD.

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Figure 2. IHC analysis of EGF using anti-EGF antibody (A00378-1).
EGF was detected in paraffin-embedded section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EGF Antibody (A00378-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of EGF using anti-EGF antibody (A00378-1).
EGF was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EGF Antibody (A00378-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 4. Sandwich ELISA - Recombinant rat EGF protein standard curve.
Use in combination with reagents from Rat EGF ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0954).

Gene/Protein Information For EGF (Source: Uniprot.org, NCBI)

Alternative Names

beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone EGF HOMG4, URG epidermal growth factor pro-epidermal growth factor|beta-urogastrone

For more info on EGF, check out the EGF Infographic

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In this infographic, you will see the following information for EGF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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