Human MPO/Myeloperoxidase ELISA Kit PicoKine®

Product Name

Human MPO/Myeloperoxidase ELISA Kit PicoKine®

SKU/Catalog Number

EK0850

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Vasoactive peptide signaling (putative): Endothelin-1 (EDN1) → endothelin receptors ETA/ETB (EDNRA/EDNRB) → GPCR/Gq–PLCβ–IP₃–Ca²⁺ cascades; roles (putative) in vasoconstriction, endothelial dysfunction, and cardiovascular remodeling. Assay context: PicoKine® sandwich ELISA; validated matrices per page—cell culture supernatants, cell lysates, human serum/plasma (heparin, EDTA); sensitivity ~0.5 pg/mL. Often profiled with vascular injury/oxidative markers such as MPO and receptor-mediated inflammation marker RAGE/AGER to contextualize cardiometabolic risk (putative).

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human MPO/Myeloperoxidase ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0850)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: A49-S745

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Standard Dilution Instructions

See datasheet of EK0850 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0850 is reactive to MPO in Human samples

Validated Sample Types

cell culture supernatants, cell lysate, serum, plasma (heparin, EDTA) , saliva and urine.

Application Guarantee

EK0850 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of MPO

Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function. The standard product used in this kit is the product of gene recombination, consisting of 697 (A49-S745) amino acids with the molecular mass of 80KDa.

Kit Components

Catalog Number	Description	Quantity
EK0850-CAP	Anti-Human MPO Pre-coated 96-well strip microplate	1
EK0850-ST	Human MPO Standard	2 vials, 20 ng/tube
EK0850-DA	Human MPO Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)	0	0.312	0.62	1.25	2.5	5	10	20
O.D.	0.015	0.117	0.2211	0.3838	0.6726	1.234	2.0	2.861

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect MPO, For serum, dilution ratio is 1:20 and concentration is 68-172 ng/ml.
For plasma, dilution ratio is 1:5 and concentration is 2.31-34.08 ng/ml..

Some articles we found to cite concentrations of MPO in samples: 25371529 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human MPO/Myeloperoxidase ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

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