Product Info Summary
SKU: | EK0850 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, cell lysate, serum, plasma (heparin, EDTA) , saliva and urine. |
Product info
Product Name
Human MPO/Myeloperoxidase ELISA Kit PicoKine®
View all Myeloperoxidase/MPO ELISA kits
SKU/Catalog Number
EK0850
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (96 Tests). Quantitate Human MPO in cell culture supernatants, cell lysate, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MPO/Myeloperoxidase ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0850)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A49-S745
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0850 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0850 is reactive to MPO in Human samples
Validated Sample Types
cell culture supernatants, cell lysate, serum, plasma (heparin, EDTA) , saliva and urine.
Application Guarantee
EK0850 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Myeloperoxidase/MPO
Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function. The standard product used in this kit is the product of gene recombination, consisting of 697 (A49-S745) amino acids with the molecular mass of 80KDa.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK0850-CAP | Anti-Human MPO Pre-coated 96-well strip microplate | 1 |
EK0850-ST | Human MPO Standard | 2 vials, 20 ng/tube |
EK0850-DA | Human MPO Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.089 | 0.280 | 0.398 | 0.602 | 0.884 | 1.128 | 1.575 | 1.926 |
Data Example Images
Click image to see more details
Human MPO PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Myeloperoxidase/MPO, For serum, dilution ratio is 1:20 and concentration is 68-172 ng/ml.
For plasma, dilution ratio is 1:5 and concentration is 2.31-34.08 ng/ml..
Some articles we found to cite concentrations of Myeloperoxidase/MPO in samples: 25371529 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MPO/Myeloperoxidase ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 580 | 2272 | 9558 | 587 | 2421 | 9230 |
Standard deviation | 42.92 | 154.49 | 401.43 | 48.13 | 191.25 | 507.65 |
CV (%) | 7.4% | 6.8% | 4.2% | 8.2% | 7.9% | 5.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Myeloperoxidase/MPO in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 580 | 586 | 560 | 663 | 597 | 39.16 | 6.5% |
Sample 2 | 2272 | 2188 | 2044 | 2304 | 2202 | 100.57 | 4.5% |
Sample 3 | 9558 | 8956 | 8970 | 10074 | 9389 | 463.9 | 4.9% |
Protein Target Info & Infographic
Gene/Protein Information For MPO (Source: Uniprot.Org, NCBI)
Gene Name
MPO
Full Name
Myeloperoxidase
Weight
83.869kDa
Superfamily
peroxidase family
Alternative Names
EC 1.11.1; EC 1.11.1.7; Lactoperoxidase; MPO; Myeloperoxidase MPO myeloperoxidase myeloperoxidase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MPO, check out the MPO Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MPO: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human MPO/Myeloperoxidase ELISA Kit PicoKine® (EK0850)
Hello CJ!
EK0850 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Bradykinin inhibits oxidative stress-induced senescence of endothelial progenitor cells through the B2R/AKT/RB and B2R/EGFR/RB signal pathways
The Burkholderia cenocepacia OmpA-like protein BCAL2958: identification, characterization, and detection of anti-BCAL2958 antibodies in serum from B. cepacia complex-infected Cystic Fibrosis patients
Mechanism of neutrophil extracellular traps generation and their role in trophoblasts apoptosis in gestational diabetes mellitus
Pentraxin 3, ischemia-modified albumin, and myeloperoxidase in predicting a cardiac damage in acute carbon monoxide poisoning
Ischemia-modified albumin is not better than creatine kinase-MB and cardiac troponin I in predicting a cardiac injury in nontraumatic subarachnoid hemorrhage
Distinct mucosal immunopathologic profiles in atopic and nonatopic chronic rhinosinusitis without nasal polyps in Central China
Distinct mucosal immunopathologic profiles in atopic and nonatopic chronic rhinosinusitis without nasal polyps in Central China
Distinct mucosal immunopathologic profiles in atopic and nonatopic chronic rhinosinusitis without nasal polyps in Central China
Bıçakçı E, Demirtaş CÖ, Çelikel Ç, Haklar G, Duman DG. Myeloperoxidase and calprotectin; Any role as non-invasive markers for the prediction of ınflammation and fibrosis in non-alcoholic steatohepatitis? Turk J Gastroenterol. 2020 Oct;31 (10) : 681-687. d
Species: Human
EK0850 usage in article: APP:ELISA, SAMPLE:HUMAN SERUM, DILUTION:NA
Peptoanaerobacter stomatis Primes Human Neutrophils and Induces Granule Exocytosis
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
16 Customer Q&As for Human MPO/Myeloperoxidase ELISA Kit PicoKine®
Question
How much serum is needed to perform the test on serum samples using EK0850?
Verified customer
Asked: 2022-07-18
Answer
For each serum sample, 100ul is sufficient to test using the Human Brorin ELISA Kit PicoKine® (EK1852).
Boster Scientific Support
Answered: 2022-07-19
Question
Have you got any information on how saliva was measured with EK0850? Are there any publications using this kit in saliva?
Verified customer
Asked: 2021-02-16
Answer
For the Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (EK0850), sample preparation using saliva is: Collect saliva using a collection device, aliquot and store samples at -20°C. The collection device should not have protein binding or filtering features. Unifortunately, we haven't found any publications using this kit in saliva yet.
Boster Scientific Support
Answered: 2021-02-19
Question
Is the detection antibody of EK0850 Monoclonal or Polyclonal?
Verified customer
Asked: 2020-12-11
Answer
The detection antibody of the Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (EK0850) is Polyclonal.
Boster Scientific Support
Answered: 2020-12-12
Question
Does EK0850 have cross-reactivity with cynomolgus monkey MPO?
Verified customer
Asked: 2020-12-11
Answer
Our lab hasn't experimentally validated cross-reactivity with cynomolgus monkey MPO using the Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (EK0850). Please see the following sequence homology: https://www.uniprot.org/blast/uniprot/B20201210216DA2B77BFBD2E6699CA9B6D1C41EB200980CE
Boster Scientific Support
Answered: 2020-12-12
Question
Does EK0850 contain recombinant protein? If yes, what is the expression system of the recombinant protein.
Verified customer
Asked: 2020-12-11
Answer
The standard of the Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (EK0850) is recombinant protein. Expression system is NSO.
Boster Scientific Support
Answered: 2020-12-12
Question
Is the capture antibody of EK0850 Monoclonal or Polyclonal?
Verified customer
Asked: 2020-12-10
Answer
The capture antibody of the Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (EK0850) is Monoclonal.
Boster Scientific Support
Answered: 2020-12-11
Question
What is the host animal for the capture antibody of EK0850?
Verified customer
Asked: 2020-12-09
Answer
The capture antibody host animal for the Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (EK0850) is mouse?
Boster Scientific Support
Answered: 2020-12-10
Question
What is the host animal for the detection antibody of EK0850?
Verified customer
Asked: 2020-12-09
Answer
The detection antibody host animal for the Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (EK0850) is goat?
Boster Scientific Support
Answered: 2020-12-10
Question
Is the standard in EK0850 purified in the active or inactive state?
Verified customer
Asked: 2020-11-10
Answer
The standard in the Human Myeloperoxidase ELISA Kit (MPO) (EK0850) was purified in the active state.
Boster Scientific Support
Answered: 2020-11-12
Question
Is there a need for additional calibrators or positive controls to run EK0850? Can vacutainers be used?
Verified customer
Asked: 2020-09-21
Answer
There is no need for any additional calibrators or positive controls to run the Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (EK0850). It is important to test positive and negative samples and no need for extra positive controls. Vacutainers can be used.
Boster Scientific Support
Answered: 2020-09-22
Question
Q: if the enzyme conjugated Myeloperoxidase antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Myeloperoxidase antigen?
Verified Customer
Verified customer
Asked: 2020-07-10
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-07-10
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-11-07
Question
Q: how can I thaw whole blood sample for Myeloperoxidase ELISA after freezing?
P. Dhar
Verified customer
Asked: 2019-09-05
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Myeloperoxidase for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-09-05
Question
Can I buy the lyophilized recombinant human MPO standard (ST0000-20) separately from the ELISA kit?
Verified Customer
Verified customer
Asked: 2018-12-31
Answer
Yes you can. We have the standard (ST0000-20): 20 ng/vial.
Boster Scientific Support
Answered: 2018-12-31
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-11-20
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-11-20
Question
Q: What is the optimal O.D. value for Myeloperoxidase ELISA kit? I performed your Myeloperoxidase ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Myeloperoxidase even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-02-02
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Myeloperoxidase ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Myeloperoxidase that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-02-02