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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysate, serum, plasma (heparin, EDTA) , saliva and urine.|
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Human MPO/Myeloperoxidase ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (96 Tests). Quantitate Human MPO in cell culture supernatants, cell lysate, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0850)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: A49-S745
312 pg/ml - 20,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0850 is reactive to MPO in Human samples
Validated Sample Types
cell culture supernatants, cell lysate, serum, plasma (heparin, EDTA) , saliva and urine.
EK0850 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0850-CAP||Anti-Human MPO Pre-coated 96-well strip microplate||1|
|EK0850-ST||Human MPO Standard||2 vials, 20 ng/tube|
|EK0850-DA||Human MPO Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human MPO PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Myeloperoxidase/MPO, Dilution ratio of 1:200, concentration in serum is around 600ng/ml.
Some articles we found to cite concentrations of Myeloperoxidase/MPO in samples: 25371529 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MPO/Myeloperoxidase ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Myeloperoxidase/MPO in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For MPO (Source: Uniprot.Org, NCBI)
EC 1.11.1; EC 18.104.22.168; Lactoperoxidase; MPO; Myeloperoxidase MPO myeloperoxidase myeloperoxidase*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on MPO, check out the MPO Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MPO: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0850 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Bıçakçı E, Demirtaş CÖ, Çelikel Ç, Haklar G, Duman DG. Myeloperoxidase and calprotectin; Any role as non-invasive markers for the prediction of ınflammation and fibrosis in non-alcoholic steatohepatitis? Turk J Gastroenterol. 2020 Oct;31 (10) : 681-687. d
EK0850 usage in article: APP:ELISA, SAMPLE:HUMAN SERUM, DILUTION:NA
Peptoanaerobacter stomatis Primes Human Neutrophils and Induces Granule Exocytosis
MicroRNA-206 is involved in the pathogenesis of ulcerative colitis via regulation of adenosine A3 receptor
The Burkholderia cenocepacia OmpA-like protein BCAL2958: identification, characterization, and detection of anti-BCAL2958 antibodies in serum from B cepacia complex-infected Cystic Fibrosis patients
Neutrophil serine proteases and their endogenous inhibitors in coronary artery ectasia patients
Bradykinin inhibits oxidative stress-induced senescence of endothelial progenitor cells through the B2R/AKT/RB and B2R/EGFR/RB signal pathways
Chen G, Yang Y, Liu M, Teng Z, Ye J, Xu Y, Cai X, Cheng X, Yang J, Hu C, Wang M, Cao P. J Ethnopharmacol. 2015 May 26;166:149-56. Doi: 10.1016/J.Jep.2015.03.027. Epub 2015 Mar 17. Banxia Xiexin Decoction Protects Against Dextran Sulfate Sodium-Ind...
Chen J, Chen X, Qin J. Brain Inj. 2011;25(3):292-9. Doi: 10.3109/02699052.2010.546824. Epub 2011 Jan 31. Effects Of Polysaccharides Of The Euphoria Longan (Lour.) Steud On Focal Cerebral Ischemia/Reperfusion Injury And Its Underlying Mechanism.
Wang W, Tang L, Li Y, Wang Y. J Neurol Sci. 2015 Jan 15;348(1-2):121-5. Doi: 10.1016/J.Jns.2014.11.018. Epub 2014 Nov 18. Biochanin A Protects Against Focal Cerebral Ischemia/Reperfusion In Rats Via Inhibition Of P38-Mediated Inflammatory Responses.
Wu Z, Ming Y, Zhang Z, Wu J, Wu Z, Yao S. Chin Med J (Engl). 2014;127(5):968-70. Edaravone Rescues The Lung By Inhibiting Lipid Peroxidation And Pro-Inflammatory Cytokines In A Rat Model.
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