Human MPO/Myeloperoxidase ELISA Kit PicoKine™

Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (96 Tests). Quantitate Human MPO in cell culture supernatants, cell lysate, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml. Cited in 10 publication(s).

Product Info Summary

SKU: EK0850
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysate, serum, plasma (heparin, EDTA) , saliva and urine.

Product Name

Human MPO/Myeloperoxidase ELISA Kit PicoKine™

See all Myeloperoxidase/MPO products

SKU/Catalog Number

EK0850

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (96 Tests). Quantitate Human MPO in cell culture supernatants, cell lysate, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0850)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A49-S745

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0850 is reactive to MPO in Human samples

Validated Sample Types

cell culture supernatants, cell lysate, serum, plasma (heparin, EDTA) , saliva and urine.

Application Guarantee

EK0850 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0850-CAP Anti-Human MPO Pre-coated 96-well strip microplate 1
EK0850-ST Human MPO Standard 2 vials, 20 ng/tube
EK0850-DA Human MPO Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251250250050001000020000
O.D.0.0890.2800.3980.6020.8841.1281.5751.926

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Myeloperoxidase/MPO, Dilution ratio of 1:200, concentration in serum is around 600ng/ml.

Some articles we found to cite concentrations of Myeloperoxidase/MPO in samples: 25371529 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human MPO/Myeloperoxidase ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5802272955858724219230
Standard deviation42.92154.49401.4348.13191.25507.65
CV (%)7.4%6.8%4.2%8.2%7.9%5.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Myeloperoxidase/MPO in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 158058656066359739.166.5%
Sample 222722188204423042202100.574.5%
Sample 3955889568970100749389463.94.9%
*number of samples for each test n=16.

Gene/Protein Information For MPO (Source: Uniprot.Org, NCBI)

Uniprot ID

P05164

Gene ID

4353

Gene Name

MPO

Full Name

Myeloperoxidase

Weight

83.869kDa

Superfamily

peroxidase family

Alternative Names

EC 1.11.1; EC 1.11.1.7; Lactoperoxidase; MPO; Myeloperoxidase MPO myeloperoxidase myeloperoxidase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MPO, check out the MPO Infographic

MPO infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MPO: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0850 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Bıçakçı E, Demirtaş CÖ, Çelikel Ç, Haklar G, Duman DG. Myeloperoxidase and calprotectin; Any role as non-invasive markers for the prediction of ınflammation and fibrosis in non-alcoholic steatohepatitis? Turk J Gastroenterol. 2020 Oct;31 (10) : 681-687. d
Species: Human
EK0850 usage in article: APP:ELISA, SAMPLE:HUMAN SERUM, DILUTION:NA

Peptoanaerobacter stomatis Primes Human Neutrophils and Induces Granule Exocytosis

MicroRNA-206 is involved in the pathogenesis of ulcerative colitis via regulation of adenosine A3 receptor

The Burkholderia cenocepacia OmpA-like protein BCAL2958: identification, characterization, and detection of anti-BCAL2958 antibodies in serum from B cepacia complex-infected Cystic Fibrosis patients

Neutrophil serine proteases and their endogenous inhibitors in coronary artery ectasia patients

Bradykinin inhibits oxidative stress-induced senescence of endothelial progenitor cells through the B2R/AKT/RB and B2R/EGFR/RB signal pathways

Chen G, Yang Y, Liu M, Teng Z, Ye J, Xu Y, Cai X, Cheng X, Yang J, Hu C, Wang M, Cao P. J Ethnopharmacol. 2015 May 26;166:149-56. Doi: 10.1016/J.Jep.2015.03.027. Epub 2015 Mar 17. Banxia Xiexin Decoction Protects Against Dextran Sulfate Sodium-Ind...

Chen J, Chen X, Qin J. Brain Inj. 2011;25(3):292-9. Doi: 10.3109/02699052.2010.546824. Epub 2011 Jan 31. Effects Of Polysaccharides Of The Euphoria Longan (Lour.) Steud On Focal Cerebral Ischemia/Reperfusion Injury And Its Underlying Mechanism.

Wang W, Tang L, Li Y, Wang Y. J Neurol Sci. 2015 Jan 15;348(1-2):121-5. Doi: 10.1016/J.Jns.2014.11.018. Epub 2014 Nov 18. Biochanin A Protects Against Focal Cerebral Ischemia/Reperfusion In Rats Via Inhibition Of P38-Mediated Inflammatory Responses.

Wu Z, Ming Y, Zhang Z, Wu J, Wu Z, Yao S. Chin Med J (Engl). 2014;127(5):968-70. Edaravone Rescues The Lung By Inhibiting Lipid Peroxidation And Pro-Inflammatory Cytokines In A Rat Model.

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15 Customer Q&As for Human MPO/Myeloperoxidase ELISA Kit PicoKine™

Question

Have you got any information on how saliva was measured with EK0850? Are there any publications using this kit in saliva?

Verified customer

Asked: 2021-02-16

Answer

For the Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (EK0850), sample preparation using saliva is: Collect saliva using a collection device, aliquot and store samples at -20°C. The collection device should not have protein binding or filtering features. Unifortunately, we haven't found any publications using this kit in saliva yet.

Boster Scientific Support

Answered: 2021-02-19

Question

Is the detection antibody of EK0850 Monoclonal or Polyclonal?

Verified customer

Asked: 2020-12-11

Answer

The detection antibody of the Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (EK0850) is Polyclonal.

Boster Scientific Support

Answered: 2020-12-12

Question

Does EK0850 have cross-reactivity with cynomolgus monkey MPO?

Verified customer

Asked: 2020-12-11

Answer

Our lab hasn't experimentally validated cross-reactivity with cynomolgus monkey MPO using the Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (EK0850). Please see the following sequence homology: https://www.uniprot.org/blast/uniprot/B20201210216DA2B77BFBD2E6699CA9B6D1C41EB200980CE

Boster Scientific Support

Answered: 2020-12-12

Question

Does EK0850 contain recombinant protein? If yes, what is the expression system of the recombinant protein.

Verified customer

Asked: 2020-12-11

Answer

The standard of the Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (EK0850) is recombinant protein. Expression system is NSO.

Boster Scientific Support

Answered: 2020-12-12

Question

Is the capture antibody of EK0850 Monoclonal or Polyclonal?

Verified customer

Asked: 2020-12-10

Answer

The capture antibody of the Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (EK0850) is Monoclonal.

Boster Scientific Support

Answered: 2020-12-11

Question

What is the host animal for the capture antibody of EK0850?

Verified customer

Asked: 2020-12-09

Answer

The capture antibody host animal for the Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (EK0850) is mouse?

Boster Scientific Support

Answered: 2020-12-10

Question

What is the host animal for the detection antibody of EK0850?

Verified customer

Asked: 2020-12-09

Answer

The detection antibody host animal for the Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (EK0850) is goat?

Boster Scientific Support

Answered: 2020-12-10

Question

Is the standard in EK0850 purified in the active or inactive state?

Verified customer

Asked: 2020-11-10

Answer

The standard in the Human Myeloperoxidase ELISA Kit (MPO) (EK0850) was purified in the active state.

Boster Scientific Support

Answered: 2020-11-12

Question

Is there a need for additional calibrators or positive controls to run EK0850? Can vacutainers be used?

Verified customer

Asked: 2020-09-21

Answer

There is no need for any additional calibrators or positive controls to run the Human MPO/Myeloperoxidase ELISA Kit PicoKine™ (EK0850). It is important to test positive and negative samples and no need for extra positive controls. Vacutainers can be used.

Boster Scientific Support

Answered: 2020-09-22

Question

Q: if the enzyme conjugated Myeloperoxidase antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Myeloperoxidase antigen?

Verified Customer

Verified customer

Asked: 2020-07-10

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-07-10

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-11-07

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-11-07

Question

Q: how can I thaw whole blood sample for Myeloperoxidase ELISA after freezing?

P. Dhar

Verified customer

Asked: 2019-09-05

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Myeloperoxidase for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2019-09-05

Question

Can I buy the lyophilized recombinant human MPO standard (ST0000-20) separately from the ELISA kit?

Verified Customer

Verified customer

Asked: 2018-12-31

Answer

Yes you can. We have the standard (ST0000-20): 20 ng/vial.

Boster Scientific Support

Answered: 2018-12-31

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2018-11-20

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-11-20

Question

Q: What is the optimal O.D. value for Myeloperoxidase ELISA kit? I performed your Myeloperoxidase ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Myeloperoxidase even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-02-02

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Myeloperoxidase ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Myeloperoxidase that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-02-02

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