Human Rage ELISA Kit PicoKine®

AGER ELISA kit for Human

Human Rage ELISA Kit PicoKine™ (96 Tests). Quantitate Human AGER in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 5 publication(s).

Product Info Summary

SKU: EK0827
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human Rage ELISA Kit PicoKine®

View all AGER ELISA kits

SKU/Catalog Number

EK0827

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Rage ELISA Kit PicoKine™ (96 Tests). Quantitate Human AGER in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Rage ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0827)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q24-A344

Sensitivity

<10 pg/ml

Assay Range

78 pg/ml - 5,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0827 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0827 is reactive to AGER in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0827 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of AGER

RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as “AGER”. AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B (NF-kappaB).

Kit Components

Catalog Number Description Quantity
EK0827-CAP Anti-Human AGER Pre-coated 96-well strip microplate 1
EK0827-ST Human AGER Standard 2 vials, 10 ng/tube
EK0827-DA Human AGER Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)078156312625125025005000
O.D.0.0370.1060.1710.2590.6090.7961.4522.004

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect AGER, Dilution ratio of 1:1, concentration in serum and plasma is 294-5224 pg/ml..

Some articles we found to cite concentrations of AGER in samples: 20726839 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Rage ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)15462330761595953344
Standard deviation10.1627.41221.4710.6532.72250.8
CV (%)6.6%4.4%7.2%6.7%5.5%7.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of AGER in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11541531411521505.243.4%
Sample 262357062759660422.963.8%
Sample 33076303530933144308739.081.2%
*number of samples for each test n=16.

Gene/Protein Information For AGER (Source: Uniprot.Org, NCBI)

Gene Name

AGER

Full Name

Advanced glycosylation end product-specific receptor

Weight

42.803kDa

Alternative Names

advanced glycosylation end product-specific receptor; AGER; RAGE isoform delta; RAGE isoform sRAGE-delta; RAGE; Receptor for advanced glycosylation end products; receptor for advanced glycosylation end-products; SCARJ1 AGER RAGE, SCARJ1, sRAGE advanced glycosylation end-product specific receptor advanced glycosylation end product-specific receptor|receptor for advanced glycation end-products variant 20

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on AGER, check out the AGER Infographic

AGER infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for AGER: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK0827 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Accumulation of advanced glycation end products (AGEs) is associated with the severity of aortic stenosis in patients with concomitant type 2 diabetes

Accumulation of Advanced Glycation End Products Involved in Inflammation and Contributing to Severe Preeclampsia, in Maternal Blood, Umbilical Blood and Placental Tissues

Long lasting protective effects of early l-arginine treatment on endothelium in an in vitro study

Rui Liu,Ying Shu,Wenhui Qi,Weili Rao,Zihan Fu,Zhenxiao Shi,Zhisheng Zhang,"Protective Effects of Almond Oil on Streptozotocin-Induced Diabetic Rats via Regulating Nrf2/HO-1 Pathway and Gut Microbiota",Journal of Food Quality,vol.2021,Article ID 5599219,14 pages,2021.https://doi.org/10.1155/2021/5599219
Species: Rat
EK0827 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Feng L, Zhu M, Zhang M, Jia X, Cheng X, Ding S, Zhu Q. Int Immunopharmacol. 2013 Feb;15(2):206-16. Doi: 10.1016/J.Intimp.2012.11.015. Epub 2012 Dec 5. Amelioration Of Compound 4,4'-Diphenylmethane-Bis(Methyl)Carbamate On High Mobility Group Box1-M...

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7 Customer Q&As for Human Rage ELISA Kit PicoKine®

Question

Q: can you suggest the dilution ratio of serum samples for detection of AGER in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

P. Wilson

Verified customer

Asked: 2020-10-31

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the AGER ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-10-31

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis AGER using the EK0827 Human AGER Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-08-28

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2019-08-28

Question

Q: how to proceed with the analysis of ELISA data? I measured AGER level in serum.

B. Johnson

Verified customer

Asked: 2019-01-26

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-01-26

Question

Q: What is the optimal O.D. value for AGER ELISA kit? I performed your AGER ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain AGER even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-12-12

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this AGER ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of AGER that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-12-12

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-08-31

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-08-31

Question

Q: which procedure should I follow in order to thaw whole blood sample for AGER ELISA after freezing?

Z. Collins

Verified customer

Asked: 2018-08-03

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test AGER for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2018-08-03

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2017-05-31

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-05-31

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