Mouse CRP / C Reactive Protein / PTX1 ELISA Kit PicoKine™

Mouse CRP / C Reactive Protein / PTX1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Crp in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 7 publication(s).

Product Info Summary

SKU: EK0977
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse CRP / C Reactive Protein / PTX1 ELISA Kit PicoKine™

See all C-Reactive Protein/CRP products

SKU/Catalog Number

EK0977

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse CRP / C Reactive Protein / PTX1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Crp in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse CRP / C Reactive Protein / PTX1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0977)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: H20-S225

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0977 is reactive to Crp in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0977 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0977-CAP Anti-Mouse Crp Pre-coated 96-well strip microplate 1
EK0977-ST Mouse Crp Standard 2 vials, 10 ng/tube
EK0977-DA Mouse Crp Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0870.2900.4240.7751.1401.4451.8862.190

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect C-Reactive Protein/CRP, Dilution ratio of 1:1000, concentration in serum is around 1ug/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse CRP / C Reactive Protein / PTX1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2441638654025517116093
Standard deviation11.7185.47307.3812.2497.52329.02
CV (%)4.8%5.2%4.7%4.8%5.7%5.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of C-Reactive Protein/CRP in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 124424528324625416.466.4%
Sample 21638173614611610161198.556.1%
Sample 365405528590160185996362.16%
*number of samples for each test n=16.

Gene/Protein Information For Crp (Source: Uniprot.Org, NCBI)

Uniprot ID

P14847

Gene ID

12944

Gene Name

Crp

Full Name

C-reactive protein

Weight

25.36kDa

Superfamily

pentraxin family

Alternative Names

C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895 Crp|AI255847|C-reactive protein, pentraxin-related|C-reactive protein|C-reactive protein, petaxin related

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Crp, check out the Crp Infographic

Crp infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Crp: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0977 has been cited in 7 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Jiao,Z.,Wen,Z.,Yang,W.,Hu,L.,& Li,J.(2021).Influence of fine particulate matter and its pure particulate fractions on pulmonary immune cells and cytokines in mice. Experimental and Therapeutic Medicine,21,662.https://doi.org/10.3892/etm.2021.10094
Species: Mouse
EK0977 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Xinxin Zhang,Yating Qin,Xiaoning Wan et al. Rosuvastatin Inhibits Atherosclerosis Development by Improving Lipid Accumulation and Polarization Conversion of Macrophages via Regulating Autophagy, 19 November 2020,PREPRINT (Version 1) available at Research
Species: Mouse
EK0977 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

In vitro modeling of COPD inflammation and limitation of p38 inhibitor - SB203580

Contribution of dermal-derived mesenchymal cells during liver repair in two different experimental models

Asgary S, Keshvari M, Sahebkar A, Hashemi M, Rafieian-Kopaei M. Arya Atheroscler. 2013 Nov;9(6):326-31. Clinical Investigation Of The Acute Effects Of Pomegranate Juice On Blood Pressure And Endothelial Function In Hypertensive Individuals.

Shi L, Song J, Zhang X, Li Y, Li H. Exp Ther Med. 2013 Aug;6(2):532-536. Epub 2013 May 28. Correlation Between The Microinflammatory State And Left Ventricular Structural And Functional Changes In Maintenance Haemodialysis Patients.

Iori E, Vinci B, Murphy E, Marescotti Mc, Avogaro A, Ahluwalia A. Plos One. 2012;7(4):E34704. Doi: 10.1371/Journal.Pone.0034704. Epub 2012 Apr 11. Glucose And Fatty Acid Metabolism In A 3 Tissue In-Vitro Model Challenged With Normo- And Hyperglyca...

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9 Customer Q&As for Mouse CRP / C Reactive Protein / PTX1 ELISA Kit PicoKine™

Question

Q: if the enzyme conjugated C-Reactive Protein antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the C-Reactive Protein antigen?

Verified Customer

Verified customer

Asked: 2020-11-14

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-11-14

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-04-21

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-04-21

Question

Q: how to proceed with the analysis of ELISA data? I have performed C-Reactive Protein level in serum.

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-11-14

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-08-28

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-08-28

Question

Q: is it okay to use citrate plasma as samples in Mouse C-Reactive Protein Picokine® ELISA Kit (Catalog # EK0977)?

A. Anand

Verified customer

Asked: 2019-05-13

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of C-Reactive Protein causing disruption of its protein structure. C-Reactive Protein may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the C-Reactive Protein ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-05-13

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

A. Garcia

Verified customer

Asked: 2018-08-04

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-08-04

Question

Q: can you suggest the dilution ratio of serum samples for detection of C-Reactive Protein in Mouse serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

W. Baker

Verified customer

Asked: 2018-05-27

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the C-Reactive Protein ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-05-27

Question

Q: how to thaw whole blood sample for C-Reactive Protein ELISA after freezing?

L. Varma

Verified customer

Asked: 2018-04-07

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test C-Reactive Protein for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-04-07

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2015-06-09

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2015-06-09

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