Overview
| Product Name |
Mouse CRP / C Reactive Protein / PTX1 ELISA Kit PicoKine™
See more C-Reactive Protein/CRP products |
| Catalog# |
EK0977 |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
Mouse CRP / C Reactive Protein / PTX1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Crp in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 10pg/ml. |
| Cite This Product |
Mouse CRP / C Reactive Protein / PTX1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0977)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Mouse |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<10 pg/ml |
| Assay Range |
156 pg/ml - 10,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: NS0; Immunogen sequence: H20-S225 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For Crp (Source: Uniprot.org, NCBI)
| Uniprot Id | P14847 |
|---|
| NCBI Gene Id | 12944 |
|---|
| Species Of This Entry | Mouse |
|---|
| Gene Name | Crp |
|---|
| Protein Name | C-reactive protein |
|---|
| Superfamily | pentraxin family |
|---|
| Alternative Names | C-Reactive Protein/CRP|C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895 |
|---|
| Molecular Weight | 25360 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Crp, check out the Crp Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Crp: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the Crp infographic
Kit Components And Assay QC Data Details Of Mouse CRP / C Reactive Protein / PTX1 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0977-CAP |
Anti-Mouse Crp Pre-coated 96-well strip microplate | 1 |
| EK0977-ST | Mouse Crp Standard | 2 vials, 10 ng/tube |
| EK0977-DA | Mouse Crp Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0977 Mouse CRP / C Reactive Protein / PTX1 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.087 | 0.290 | 0.424 | 0.775 | 1.140 | 1.445 | 1.886 | 2.190 |
We measured random samples of Mouse CRP / C Reactive Protein / PTX1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 244 | 1638 | 6540 | 255 | 1711 | 6093 |
| Standard deviation | 11.71 | 85.47 | 307.38 | 12.24 | 97.52 | 329.02 |
| CV (%) | 4.8% | 5.2% | 4.7% | 4.8% | 5.7% | 5.4% |
We measured the performance consistency of Mouse CRP / C Reactive Protein / PTX1 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 244 | 245 | 283 | 246 | 254 | 16.46 | 6.4% |
| Sample 2 | 1638 | 1736 | 1461 | 1610 | 1611 | 98.55 | 6.1% |
| Sample 3 | 6540 | 5528 | 5901 | 6018 | 5996 | 362.1 | 6% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 6
Xinxin Zhang,Yating Qin,Xiaoning Wan et al. Rosuvastatin Inhibits Atherosclerosis Development by Improving Lipid Accumulation and Polarization Conversion of Macrophages via Regulating Autophagy, 19 November 2020,PREPRINT (Version 1) available at Research
Species: Mouse
EK0977 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA |
View Publication |
| In vitro modeling of COPD inflammation and limitation of p38 inhibitor - SB203580 |
PubMed ID 27199554 |
| Contribution of dermal-derived mesenchymal cells during liver repair in two different experimental models |
PubMed ID 27126764 |
| Asgary S, Keshvari M, Sahebkar A, Hashemi M, Rafieian-Kopaei M. Arya Atheroscler. 2013 Nov;9(6):326-31. Clinical Investigation Of The Acute Effects Of Pomegranate Juice On Blood Pressure And Endothelial Function In Hypertensive Individuals. |
PubMed ID 24575134 |
| Shi L, Song J, Zhang X, Li Y, Li H. Exp Ther Med. 2013 Aug;6(2):532-536. Epub 2013 May 28. Correlation Between The Microinflammatory State And Left Ventricular Structural And Functional Changes In Maintenance Haemodialysis Patients. |
PubMed ID 24137221 |
| Iori E, Vinci B, Murphy E, Marescotti Mc, Avogaro A, Ahluwalia A. Plos One. 2012;7(4):E34704. Doi: 10.1371/Journal.Pone.0034704. Epub 2012 Apr 11. Glucose And Fatty Acid Metabolism In A 3 Tissue In-Vitro Model Challenged With Normo- And Hyperglyca... |
PubMed ID 22509346 |
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Questions and answers from customers related to EK0977 Mouse CRP / C Reactive Protein / PTX1 ELISA Kit PicoKine™
9 Related Questions
Question
Q: if the enzyme conjugated C-Reactive Protein antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the C-Reactive Protein antigen?
Verified Customer
Asked: 2020-11-14
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-11-14
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Asked: 2020-04-21
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-04-21
Question
Q: how to proceed with the analysis of ELISA data? I have performed C-Reactive Protein level in serum.
Verified Customer
Asked: 2019-11-14
Boster Scientific Support
Answered: 2019-11-14
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Asked: 2019-08-28
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-08-28
Question
Q: is it okay to use citrate plasma as samples in Mouse C-Reactive Protein Picokine® ELISA Kit (Catalog # EK0977)?
A. Anand
Asked: 2019-05-13
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of C-Reactive Protein causing disruption of its protein structure. C-Reactive Protein may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the C-Reactive Protein ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-05-13
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
A. Garcia
Asked: 2018-08-04
Boster Scientific Support
Answered: 2018-08-04
Question
Q: can you suggest the dilution ratio of serum samples for detection of C-Reactive Protein in Mouse serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
W. Baker
Asked: 2018-05-27
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the C-Reactive Protein ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-05-27
Question
Q: how to thaw whole blood sample for C-Reactive Protein ELISA after freezing?
L. Varma
Asked: 2018-04-07
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test C-Reactive Protein for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-04-07
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Asked: 2015-06-09
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2015-06-09
