Human IL-6 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Product Name

Human IL-6 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all IL6 products

SKU/Catalog Number

EZ0410

Size

For 5 plates, 96 wells each, reagents only, plates are not included

*Question: How many samples can I assay/run in this kit?

Description

Cytokine-mediated signaling (putative; GO:0019221) and acute-phase induction (putative); clinical utility (putative): inflammation monitoring. Assay context: DIY antibody pairs for sandwich ELISA; human matrices include serum/plasma (heparin/EDTA/citrate) and cell supernatants; assay range 4.69–300 pg/mL; no detectable cross-reactivity with other relevant proteins (from product page). Often profiled with TNF-α, CRP, and LBP in inflammatory panels (putative).

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human IL-6 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0410)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: P29-M212

Assay Range

4.69 pg/ml-300 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0410 is reactive to IL6 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EZ0410 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number	Description	Quantity	Storage of opened/reconstituted material
EZ0410-CA	Mouse anti- human IL6 monoclonal antibody (Capture Antibody)	500 µl, 0.4 mg/mL (recommended dilution 1:100)	Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0410-DA	Biotinylated goat anti- human IL6 polyclonal antibody (Detection Antibody)	500 µl, 3.9 µg/mL (recommended dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	500 µl (recommended dilution 1:100)
EK0410-ST	Lyophilized recombinant human IL6 standard	10 ng/tube×3	Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	4.69	9.38	18.75	37.5	75	150	300
O.D.	0.031	0.091	0.127	0.222	0.513	0.749	1.186	1.997

Data Example Images

Gene/Protein Information For IL6 (Source: Uniprot.Org, NCBI)

Alternative Names

cDNA FLJ61423, highly similar to Interleukin-6 ;

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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