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Product Info Summary
| SKU: | AR0024 |
|---|---|
| Size: | 1 pack |
Product info
Overview
| Product Name | Citrate Buffer Pack |
|---|---|
| SKU/Catalog Number | AR0024 |
| Pack Size | 2000mL/pack | Form | Dry-blend powder, sufficient to make 2000mL of Citrate Buffer |
| Volume of Final Solution | 2000 mL |
| pH | 6 |
| Buffer Contents | 3g C6H8O7.H2, 20g C6H5Na3O7.2H2O |
| Composition | Sodium citrate.H2O |
| Storage | Store at room temperature. It is stable at room temperature for one year. |
| Application | IHC-P, HIER, PEFF tissues *Our Boster Guarantee covers the use of this product in the above tested applications. |
| Equivalent | Abcam (Product No. ab64214, ab64236) |
| Description | Boster’s Citrate buffer solution is an IHC antigen retrieval reagent used in heat induced epitope retrieval (HIER) for recovery of antigens masked by cross-linking during fixation. |
| Cite This Product | Citrate Buffer Pack (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR0024) |
Chemical Information
| CAS Number | Trisodium citrate | 68-04-2 |
|---|---|---|
| Citric acid | 77-92-9 | |
| Molecular Weight | Trisodium citrate | 258.07 |
| Citric acid | 192.123 | |
| Molecular Formula | Trisodium citrate | C6H5Na3O7 |
| Citric acid | C6H8O7 | |
| Chemical Sources | Trisodium citrate | ChEMBL: ChEMBL1355 |
| ChemSpider: 5989 | ||
| PubChem: 6224 | ||
| Citric acid | ChEMBL: ChEMBL1261 | |
| ChemSpider: 305 | ||
| PubChem: 311 |
Usage and Handling
See the IHC protocol for more specific usage instructions.
Certain antigens may utilize a combination of retrieval methods. Antigen retrieval may not be required for frozen cross section as cross links are formed during formalin fixation.
Advice: In order to achieve sufficient retrieval or avoid tissue destruction or over digestion, appropriate temperature, pH, buffer concentration, incubation times, and other conditions should be carefully selected for each particular antigen, antibody, or tissue type after preliminary experimentation by the researcher.
Note: All of Boster's products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use.
Product Images
Validation Images & Assay Conditions
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Chemical structure of Sodium Citrate
Specific Publications For AR0024
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2 Customer Q&As for Citrate Buffer Powder
Question
What is the weight AR0024 supplied in grams?
Verified customer
Asked: 2020-11-30
Answer
For the Citrate Buffer Powder (AR0024), the weight of the powder supplied is 23g. Buffer Contents: 3g C6H8O7.H2, 20g C6H5Na3O7.2H2O
Boster Scientific Support
Answered: 2020-11-30
Question
Can AR0024 be used for the antigen retrieval in the PA2079 protocol as well?
Verified customer
Asked: 2019-04-18
Answer
Yes, Citrate Buffer Powder AR0024 can be used for the antigen retrieval in the Anti-VE-Cadherin-5 CDH5-Antibody (PA2079) protocol.
Boster Scientific Support
Answered: 2019-04-19
