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Product Info Summary
| SKU: | M06819-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Vertebrates |
| Host: | Rabbit |
| Application: | ChIP, ELISA, Luminex |
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Product info
Product Name
Anti-Trimethyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM340
SKU/Catalog Number
M06819-2
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Trimethyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM340 (Catalog # M06819-2). Tested in ChIP, ELISA, Luminex applications. This antibody reacts with Human, Vertebrates.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Trimethyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM340 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06819-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM340
Isotype
Rabbit IgG
Immunogen
A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys4)
Cross-reactivity
This antibody reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross-reactivity with monomethylated Lysine 4 (K4me1) or dimethylated Lysine 4 (K4me3), or other methylation in histone H3.
Reactive Species
M06819-2 is reactive to H3F3A in Human, Vertebrates
Calculated molecular weight
15.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06819-2 is guaranteed for ChIP, ELISA, Luminex Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Chromatin IP: 1 ug/mL – 5 ug/mL
ELISA: 1 ug/mL – 10 ug/mL
Luminex: 0.5 ug/mL – 2 ug/mL.
Validation Images & Assay Conditions
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ELISA result showing specificity
ELISA of Designer Nucleosomes (dNucs) (Recombinant Human Nucleosome with H3 K4 Modified), using anti-H3K4me3 rabbit monoclonal antibody clone RM340 (10 g/mL).
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ChIP result
SNAP-ChIP TM / qPCR using anti-H3K4me3 rabbit monoclonal antibody clone RM340. Antibody (3 g at 17 g/mL) was tested in native ChIP with 3 g HEK-293 chromatin (~1x106 cells). Specificity (left Y-axis
Specific Publications For Anti-Trimethyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM340 (M06819-2)
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