Product Info Summary
| SKU: | AZQ38PL0 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Zebrafish FGF20A Antibody
SKU/Catalog Number
AZQ38PL0
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish FGF20A Antibody catalog # AZQ38PL0. Tested in IHC applications. This antibody reacts with Zebrafish.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish FGF20A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ38PL0)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish FGF20A recombinant protein (Position: S10-S208).
Reactive Species
AZQ38PL0 is reactive to FGF20A in Zebrafish
Background of FGF20A
Predicted to enable fibroblast growth factor receptor binding activity and growth factor activity. Acts upstream of or within anatomical structure homeostasis; fin regeneration; and regulation of bone remodeling. Predicted to be active in cytoplasm and extracellular space. Is expressed in caudal fin; dermatocranium; inter-frontal joint; and kidney. Human ortholog(s) of this gene implicated in renal agenesis. Orthologous to human FGF20 (fibroblast growth factor 20).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ38PL0 is guaranteed for IHC Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Immunohistochemistry(Paraffin-embedded Section) | 2-5 μg/ml | Zebrafish |
Validation Images & Assay Conditions
Click image to see more details
IHC analysis of FGF20A using anti-FGF20A antibody (AZQ38PL0).
FGF20A was detected in a paraffin-embedded section of zebrafish bone tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGF20A Antibody (AZQ38PL0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of FGF20A using anti-FGF20A antibody (AZQ38PL0).
FGF20A was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGF20A Antibody (AZQ38PL0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of FGF20A using anti-FGF20A antibody (AZQ38PL0).
FGF20A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGF20A Antibody (AZQ38PL0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish FGF20A Antibody (AZQ38PL0)
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