Product Info Summary
| SKU: | DZ41027 |
|---|---|
| Size: | 200 μl/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IF, IHC |
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Product info
Product Name
Anti-zebrafish Ptprq Antibody
SKU/Catalog Number
DZ41027
Size
200 μl/vial
Form
Liquid
Description
Boster Bio Anti-Ptprq Antibody catalog # DZ41027. This antibody reacts with zebrafish.
Storage & Handling
At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Cite This Product
Anti-zebrafish Ptprq Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ41027)
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Reactive Species
DZ41027 is reactive to ptprq in Zebrafish
Application & Images
Applications
DZ41027 is guaranteed for IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5μg/ml
Immunofluorescence, 4 μg/ml
Positive Control
IHC: zebrafish intestines tissue, zebrafish inner ear tissue, zebrafish kidney tissue, zebrafish ovary tissue
ICC/IF: zebrafish embryos tissue
Validation Images & Assay Conditions
Click image to see more details
IHC analysis of Ptprq using anti-Ptprq antibody (DZ41027).
Ptprq was detected in paraffin-embedded section of zebrafish intestines tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Ptprq Antibody (DZ41027) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of Ptprq using anti-Ptprq antibody (DZ41027).
Ptprq was detected in paraffin-embedded section of zebrafish inner ear tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Ptprq Antibody (DZ41027) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of Ptprq using anti-Ptprq antibody (DZ41027).
Ptprq was detected in paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Ptprq Antibody (DZ41027) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of Ptprq using anti-Ptprq antibody (DZ41027).
Ptprq was detected in paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Ptprq Antibody (DZ41027) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IF analysis of Ptprq using anti-Ptprq antibody (DZ41027).
Ptprq was detected in a paraffin-embedded section of zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 4 μg/mL rabbit anti-Ptprq Antibody (DZ41027) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
IF analysis of Ptprq using anti-Ptprq antibody (DZ41027).
Ptprq was detected in a paraffin-embedded section of zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 4 μg/mL rabbit anti-Ptprq Antibody (DZ41027) overnight at 4°C.DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
IF analysis of Ptprq using anti-Ptprq antibody (DZ41027).
Ptprq was detected in a paraffin-embedded section of zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 4 μg/mL rabbit anti-Ptprq Antibody (DZ41027) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
IF analysis of Ptprq using anti-Ptprq antibody (DZ41027).
Ptprq was detected in a paraffin-embedded section of zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 4 μg/mL rabbit anti-Ptprq Antibody (DZ41027) overnight at 4°C.DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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