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Product Info Summary
|Description:||Transient overexpression lysate of chemokine (C-C motif) ligand 5 (CCL5)|
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RANTES (CCL5) (NM_002985) Human Over-expression Lysate
Transient overexpression lysate of chemokine (C-C motif) ligand 5 (CCL5)
Storage & Handling
Cite This Product
RANTES (CCL5) (NM_002985) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006352)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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RANTES (CCL5) Human Over-expression Lysates NM_002985.
Western blot validation of overexpression lysate (LS006352) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CCL5 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for CCL5 (Source: Uniprot.org, NCBI)
C-C motif chemokine 5
intercrine beta (chemokine CC) family
CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES CCL5 D17S136E, RANTES, SCYA5, SIS-delta, SISd, TCP228, eoCP C-C motif chemokine ligand 5 C-C motif chemokine 5|T-cell specific protein p288|beta-chemokine RANTES|chemokine (C-C motif) ligand 5|eosinophil chemotactic cytokine|regulated upon activation, normally T-expressed, and presumably secreted|small inducible cytokine subfamily A (Cys-Cys), member 5*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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