Rat BDNF ELISA Kit PicoKine®

Bdnf ELISA kit for Rat

Rat BDNF ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Bdnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml. Cited in 10 publication(s).

Product Info Summary

SKU: EK0308
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate).

Product Name

Rat BDNF ELISA Kit PicoKine®

View all Bdnf ELISA kits

SKU/Catalog Number

EK0308

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat BDNF ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Bdnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rat BDNF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0308)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: H129-R247

Sensitivity

<15 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0308 is reactive to Bdnf in Rat samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0308 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Bdnf

Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury.

Kit Components

Catalog Number Description Quantity
EK0308-CAP Anti-Rat Bdnf Pre-coated 96-well strip microplate 1
EK0308-ST Rat Bdnf Standard 2 vials, 10 ng/tube
EK0308-DA Rat Bdnf Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0250.0740.1010.1600.2870.5631.2511.916

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat BDNF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)8829290684273977
Standard deviation4.416.0670.664.219.1177.18
CV (%)5%5.5%7.8%5%7%7.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Bdnf in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1889890102945.726%
Sample 22922752662812789.443.3%
Sample 390699390193593336.583.9%
*number of samples for each test n=16.

Gene/Protein Information For Bdnf (Source: Uniprot.Org, NCBI)

Uniprot ID

P23363

Gene ID

24225

Gene Name

Bdnf

Full Name

Brain-derived neurotrophic factor

Weight

28.109kDa

Superfamily

NGF-beta family

Alternative Names

Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin Bdnf|brain-derived neurotrophic factor|brain-derived neurotrophic factor|brain derived neurothrophic factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Bdnf, check out the Bdnf Infographic

Bdnf infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Bdnf: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0308 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Expression of neurotrophic factors in injured spinal cord after transplantation of human-umbilical cord blood stem cells in rats.

Study of brain-derived neurotrophic factor gene transgenic neural stem cells in the rat retina

Resveratrol, a SIRT1 Activator, Ameliorates MK-801-Induced Cognitive and Motor Impairments in a Neonatal Rat Model of Schizophrenia

Long-Term Running Exercise Delays Age-Related Changes in White Matter in Rats

Study on the mechanism of TMRK electroacupuncture in repairing synaptic plasticity in amygdala and hippocampus to relieve fear memory in PTSD rats

Centella asiatica (Gotu kola) ethanol extract up-regulates hippocampal brain-derived neurotrophic factor (BDNF), tyrosine kinase B (TrkB) and extracellular signal-regulated protein kinase 1/2 (ERK1/2) signaling in chronic electrical stress model in rats

Transplantation of BDNF Gene Recombinant Mesenchymal Stem Cells and Adhesive Peptide-modified Hydrogel Scaffold for Spinal Cord Repair

Triptolide improves neurobehavioral functions, inflammation, and oxidative stress in rats under deep hypothermic circulatory arrest

Effects of single-dose neuropeptide Y on levels of hippocampal BDNF, MDA, GSH, and NO in a rat model of pentylenetetrazole-induced epileptic seizure

GT1b functions as a novel endogenous agonist of toll‐like receptor 2 inducing neuropathic pain

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19 Customer Q&As for Rat BDNF ELISA Kit PicoKine®

Question

Is this kit compatible with tissue homogenates taken from Wistar rats hippocampus and hypothalamus?

Verified customer

Asked: 2021-12-15

Answer

Our lab hasn't worked on tissue lysates with EK0308. Endogenous biotin in tissue homogenates might introduce false positive signal. We recommend the customer to run a pilot test as described below to confirm the affect of endogeous biotin first. Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, the customer can work on the tissue sample by using the kit. Please see the following suggested protocol. Procedure for Lysis of Tissues Note: Pre-chill an appropriate volume of IP Lysis Buffer at 4 ˚C. If desired, add protease inhibitor and phosphatase inhibitor to the lysis buffer immediately before use. 1.Place the fresh tissue into chilled PBS and rinse several times. Mince the tissue into small pieces. 2.Add IP lysis buffer to the tissue. We recommend the customer to use lysis buffer from Thermofisher. https://www.thermofisher.cn/order/catalog/product/87788?SID=srch-srp-87788#/87788?SID=srch-srp-87788 3.Homogenize for several minutes with a glass homogenizer at high speed until no tissue chunks remain. 4.Incubate on ice for 10 minutes. 5.Centrifuge at ~13000 x g for 10 minutes at 4°C. 6.Transfer supernatant to a new tube for further analysis.

Boster Scientific Support

Answered: 2021-12-16

Question

Would you predict that this kit would work with tissue lysates? And if so, do you have a recommended protocol for tissue lysis that would be compatible with the kit?

Verified customer

Asked: 2021-12-13

Answer

Our lab hasn't worked on tissue lysates with EK0308. Endogenous biotin in tissue homogenates might introduce false positive signals. We recommend running a pilot test as described below to confirm the effect of endogenous biotin first. Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, you can work on the tissue sample by using the kit. Please see the following suggested protocol. Procedure for Lysis of Tissues: Note: Pre-chill an appropriate volume of IP Lysis Buffer at 4 ˚C. If desired, add protease inhibitor and phosphatase inhibitor to the lysis buffer immediately before use. 1.Place the fresh tissue into chilled PBS and rinse several times. Mince the tissue into small pieces. 2.Add IP lysis buffer to the tissue. We recommend the customer to use lysis buffer from Thermofisher. https://www.thermofisher.cn/order/catalog/product/87788?SID=srch-srp-87788#/87788?SID=srch-srp-87788 3.Homogenize for several minutes with a glass homogenizer at high speed until no tissue chunks remain. 4.Incubate on ice for 10 minutes. 5.Centrifuge at ~13000 x g for 10 minutes at 4°C. 6.Transfer supernatant to a new tube for further analysis.

Boster Scientific Support

Answered: 2021-12-15

Question

Q: is it okay to use EDTA plasma as samples in Rat Bdnf Picokine® ELISA Kit (Catalog # EK0308)?

T. Kulkarni

Verified customer

Asked: 2020-11-18

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Bdnf causing degradation of its protein structure. Bdnf may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Bdnf ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-11-18

Question

Q: Can Bdnf ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-11-10

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-11-10

Question

How is the protein in the expression system of EK0308 standard purified?

Verified customer

Asked: 2020-10-22

Answer

The protein in the Rat BDNF ELISA Kit PicoKine™ (EK0308) standard is purified through nickel-affinity chromatography column.

Boster Scientific Support

Answered: 2020-10-22

Question

Does EK0308 use baculovirus?

Verified customer

Asked: 2020-10-06

Answer

The standard in the Rat BDNF ELISA Kit PicoKine™ (EK0308) used baculovirus.

Boster Scientific Support

Answered: 2020-10-20

Question

Is wash treatment process needed when using EK0308 or not?

Verified customer

Asked: 2020-03-03

Answer

For Rat BDNF ELISA Kit PicoKine EK0308, our lab technicians found there wasn't any significant difference in result values between washing and without washing the plate after incubating samples/standard and before addition of the detection antibody. It is to make the protocol easy and fast.

Boster Scientific Support

Answered: 2020-03-10

Question

Q: can you suggest the dilution ratio of serum samples for detection of Bdnf in Rat plasma? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2020-02-15

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Bdnf ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-02-15

Question

Does EK0308 recognize only mature BDNF? Or does this kit recognize both of mature BDNF and proBDNF?

Verified Customer

Verified customer

Asked: 2020-02-06

Answer

EK0308 recognizes both mature BDNF and proBDNF

Boster Scientific Support

Answered: 2020-02-06

Question

Will EK0308 be able to cross-react with human samples?

Verified Customer

Verified customer

Asked: 2019-11-06

Answer

EK0308 is able to cross-react with human.

Boster Scientific Support

Answered: 2019-11-06

Question

Q: What is the optimal O.D. value for Bdnf ELISA kit? I performed your Bdnf ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Bdnf even though the O.D. values are not very high?

P. Allen

Verified customer

Asked: 2019-11-02

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Bdnf ELISA kit to have sensitivity of 15pg/ml, that means the minimum amount of Bdnf that can be declared/interpreted as positive by the above standard is 15pg/ml.

Boster Scientific Support

Answered: 2019-11-02

Question

Will polyethylene and polypropylene adsorb BDNF?

Verified Customer

Verified customer

Asked: 2019-09-25

Answer

Polypropylene will not adsorb BDNF. We are uncertain if Polypropylene will adsorb BDNF.

Boster Scientific Support

Answered: 2019-09-25

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Bdnf using the EK0308 Rat Bdnf Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-07-20

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2019-07-20

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

T. Bhasin

Verified customer

Asked: 2019-02-18

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-02-18

Question

Q: if the enzyme conjugated Bdnf antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Bdnf antigen?

Verified Customer

Verified customer

Asked: 2018-11-24

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-11-24

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-04-30

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-04-30

Question

My samples have been stored as lysates frozen in SDS lysis buffer. Are my samples compatible with this kit?

Verified Customer

Verified customer

Asked: 2018-04-19

Answer

SDS may affect the kit's performance, and therefore we don't suggest you keep your lysates in your SDS lysis buffer.

Boster Scientific Support

Answered: 2018-04-19

Question

Q: how to analyze ELISA data? I measured Bdnf level in plasma.

Verified Customer

Verified customer

Asked: 2017-12-16

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2017-12-16

Question

Today we got an inquiry regarding to your #EK0308 rat BDNF ELISA kit, the user would liketo know if a platelet-poor plasma is necessary to be prepared while assay plasma sample. In addition, she also would like to know if you have suggested dilution factor for analyzing plasma sample.

Verified Customer

Verified customer

Asked: 2016-07-07

Answer

For EK0308 rat BDNF ELISA kit, platelet-poor plasma should be collected for measurement. The suggested dilution factor for analyzing plasma sample is 1:50. All plasma samples for EK0307, EK0308 and EK0309 should be prepared as platelet-poor plasma before assay. Please see the following suggested protocol to prepare platelet-poor plasma sample. Collect plasma on ice using EDTA or heparin as an anticoagulant. Centrifuge at 2-8

Boster Scientific Support

Answered: 2016-07-07

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