Immunohistochemistry (IHC) & Multiplex IF Services

Study Context: Mouse brain coronal sections | Human IgG tracking | Peroxidase labeling | Regional quantification

The Problem: A postdoc from Stanford University needed to visualize human IgG distribution in mouse brain tissue following intracerebroventricular injection. Coronal sectioning, regional tissue targeting, and signal quantification were required.

Our Approach: We performed precise coronal brain sectioning and optimized peroxidase-labeled anti-human IgG staining, with quantification options for signal intensity across sections.

Outcome: We prepared high-quality paraffin-embedded tissue, optimized peroxidase-labeled staining, and generated quantitative data compatible with complementary platforms such as Flow Cytometry.

Study Context: Human tissue | 3 fixatives (NBF, ethanol, proprietary) | Multiple timepoints | Morphology + DNA integrity

The Problem: A global lab group testing a new formalin-free fixative wanted to compare tissue quality and IHC performance vs. standard 10% NBF and 70% ethanol across multiple timepoints.

Our Approach: We processed matched human tissues with three fixatives, performed IHC staining, and documented staining quality, cell morphology, and DNA integrity results at various fixation durations.

Outcome: The client used the findings to support a white paper and validate their product for broader diagnostic applications, citing our standardized tissue processing and unbiased results.

Study Context: 5xFAD mouse model | 4-marker multiplex (GFAP, Iba1, Amyloid-beta, Tau) | Frozen tissue | Bundled with ELISA

The Problem: A PhD researcher from UT Southwestern studying Alzheimer's in a 5xFAD mouse model needed multiplex immunohistochemistry (IHC) for several brain markers (GFAP, Iba1, Amyloid-beta, Tau) and simultaneous ELISA data for cytokines and amyloid isoforms.

Our Approach: We used our in-house validated antibodies and multiplex staining protocols on frozen tissues, paired with cytokine/amyloid ELISA using our bundled service.

Outcome: The client saved time and cost through bundled services, avoided delays in antibody validation, and received complete, publication-ready datasets.

Study Context: Rare tumor type | Limited irreplaceable samples | Tissue microarray validation | Risk-minimized handling

The Problem: A translational research group at the University of Minnesota was working with extremely limited samples from a rare tumor type. Internal staining attempts had failed, and any further mistakes would mean losing irreplaceable material.

Our Approach: We applied careful handling protocols, used validated reagents, and ran optimization passes on parallel tissue to protect the primary sample including optional tissue microarrays for method verification. Our experience with rare sample types helped reduce the risk of data loss.

Outcome: High-quality, interpretable staining results were delivered without sample loss—enabling the team to move forward with their publication and grant submission.

Study Context: Limited imaging access | No confocal microscope | Publication-quality requirements | Whole slide scanning

The Problem: A PI from University of California, Riverside lacked access to modern imaging systems—no confocal microscope and outdated brightfield optics—making it impossible to publish high-resolution IHC data.

Our Approach: We performed high-resolution whole slide fluorescence scanning using our Leica system with 40X and multi-focus imaging options, generating publication-ready digital slides with crisp signal capture.

Outcome: The PI received high-quality images that met journal standards, allowing the team to finalize figures and complete their manuscript without buying new equipment.

Study Context: Tumor microenvironment | 10-marker multiplex IHC | Signal separation optimization | Cancer immunology

The Problem: A cancer immunology lab needed a 10-marker IHC panel to profile the tumor microenvironment. They had experience with 3-plex, but higher multiplexing was beyond their capabilities.

Our Approach: We developed a custom 10-plex multiplex IHC protocol using our in-house validated antibodies and optimized signal separation workflows, cutting down development time by weeks.

Outcome: The lab received fully stained, multiplexed slides and analysis-ready data within their timeline—saving months of trial-and-error optimization and ensuring reliable signal detection.

Study Context: 2-week deadline | Grant application supporting data | Validated antibodies | Leica Bond automation

The Problem: A PI from UCSF had two weeks to gather supporting IHC data for a time-sensitive grant application. Their students were overwhelmed, and no one had time to troubleshoot protocols from scratch.

Our Approach: We quickly reviewed the study design, procured validated antibodies, and used our Leica Bond automation to deliver high-quality staining on a tight timeline.

Outcome: The client met their grant deadline with solid, interpretable images—without pulling students