Source: Biocompare.com
| SKU | EK0397 |
|---|---|
| Applications | Detection of secreted IL-2 in media of Jurkat T cells |
| Sample | Jurkat T cell media (RPMI, 10% FBS, 1% pen/strep) |
| Detection | -- |
"I was using this kit to detect IL-2 secretion from Jurkat T-cells upon activation with PMA and PHA. I followed the protocol exactly. I had extremely low levels of IL-2 in my experiment, which were just above background. In this case, it was essential for me to use the standards and blanks to ensure that my samples were above background. All components come with the kit, unlike some other kits from other companies in which only some of the necessary components are provided. This is a great kit that is a bit expensive, but worth the cost for an easy-to-use and sensitive assay."
