Overview
| Product Name |
14-3-3 zeta (Phospho-Ser58) Colorimetric Cell-Based ELISA Kit
See more YWHAZ products |
| Catalog# |
EKC1934 |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
The 14-3-3 zeta (Phospho-Ser58) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor 14-3-3 zeta (Phospho-Ser58) protein expression profile in cells. The kit can be used for measuring the relative amounts of 14-3-3 zeta (Phospho-Ser58) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on 14-3-3 zeta (Phospho-Ser58). |
| Cite This Product |
14-3-3 zeta (Phospho-Ser58) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1934)
|
Product Specs
| Reactivity/Species |
Human, Mouse, Rat |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
Cells |
| Sensitivity |
>5000 cells |
| Assay Range |
> 5000 Cells |
| Pack Size |
1 kit, containing 2 plates and all necessary reagents |
Gene/Protein Basic Information For YWHAZ (Source: Uniprot.org, NCBI)
| Uniprot Id | P63104 |
|---|
| NCBI Gene Id | 7534 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | YWHAZ |
|---|
| Protein Name | 14-3-3 protein zeta/delta |
|---|
| Superfamily | 14-3-3 family |
|---|
| Alternative Names | YWHAZ|1433 zeta; 14-3-3 zeta; KCIP-1; KCIP-1MGC126532,14-3-3-zeta; MGC111427; MGC138156; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, deltapolypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zetapolypeptide; YWHAZ; zeta polypeptide |
|---|
| Molecular Weight | 27745 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on YWHAZ, check out the YWHAZ Infographic
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Kit Components And Assay QC Data Details Of 14-3-3 zeta (Phospho-Ser58) Colorimetric Cell-Based ELISA Kit
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Reagent |
Quantity |
Container |
| 96-Well Cell Culture Clear-Bottom Microplate |
2 Plates |
- |
| 10x TBS |
24 ml (10x) |
Clear |
| Quenching Buffer |
24 ml (1x) |
Clear |
| Blocking Buffer |
50 ml (1x) |
Clear |
| 15x Wash Buffer |
50 ml (15x) |
Clear |
| 100x Anti-YWHAZ (Phospho-specific) Antibody (Rabbit Polyclonal) |
60 µl (100x) |
Purple |
| 100x Anti-YWHAZ Antibody (Rabbit Polyclonal) |
60 µl (100x) |
Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) |
60 µl (100x) |
Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody |
12ml (1x) |
Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody |
12ml (1x) |
Glass |
| Primary Antibody Diluent |
12 ml (1x) |
Clear |
| Ready-to-Use Substrate |
12 ml (1x) |
Brown |
| Stop Solution |
12 ml (1x) |
Clear |
| Crystal Violet Solution |
12ml (1x) |
Glass |
| SDS Solution |
24 ml (1x) |
Clear |
| Adhesive Plate Seals |
2 Seals |
- |
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Western blot analysis of extracts from NIH-3T3 cells treated with UV 30'
Specific Protocols
The protocol for this particular product is in the datasheet. Download the datasheet in the datasheet link at the top of the page.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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