14-3-3 protein zeta/delta Antibodies

AND YWHAZ ELISA kits, 14-3-3 protein zeta/delta Proteins

Many biological assays use antibodies to detect 14-3-3 protein zeta/delta, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with 14-3-3 protein zeta/delta in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop 14-3-3 protein zeta/delta antibodies...

We validate the specificity of these antibodies to 14-3-3 protein zeta/delta by testing them on tissues known to express YWHAZ positively and negatively. Browse below to find the YWHAZ antibody that suites your experiment. We have 15 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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YWHAZ Infographic by Boster Bio

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Anti-14-3-3 zeta/delta/YWHAZ Antibody Picoband®

5 Q&As

Western blot analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (A01141-1)

IF analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (A01141-1)

Human, Mouse, Rat

IF, ICC, WB

$370
Cat # A01141-1
100 μg/vial

14-3-3 beta/zeta Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from NIH-3T3 cells

Human, Mouse, Rat

ELISA

$480
Cat # EKC1001
1 kit, containing one 96-well plate and all necessary reagents

14-3-3 zeta Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from 293 cells

Human, Mouse, Rat

ELISA

$480
Cat # EKC1002
1 kit, containing one 96-well plate and all necessary reagents

14-3-3 zeta (Phospho-Ser58) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from NIH-3T3 cells treated with UV 30

Human, Mouse, Rat

ELISA

$540
Cat # EKC1934
1 kit, containing two 96-well plates and all necessary reagents

14-3-3 zeta/delta (Phospho-Thr232) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from Jurkat cells treated with UV 15

Human, Mouse, Rat

ELISA

$540
Cat # EKC2182
1 kit, containing two 96-well plates and all necessary reagents

14-3-3 beta/zeta (Phospho-Ser184/186) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from RAW264

Human, Mouse, Rat

ELISA

$540
Cat # EKC2541
1 kit, containing two 96-well plates and all necessary reagents

Anti-14-3-3 zeta/delta/YWHAZ Antibody Picoband® (monoclonal, 6G5)

Western blot analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (M01141)

Flow Cytometry analysis of PC-3 cells using anti-14-3-3 zeta/delta antibody (M01141)

Flow Cytometry analysis of SiHa cells using anti-14-3-3 zeta/delta antibody (M01141)

Human, Monkey, Mouse, Rat

Flow Cytometry, WB

$370
Cat # M01141
100 μg/vial

Anti-14-3-3 zeta/delta/YWHAZ Antibody Picoband® (monoclonal, 6H7)

Western blot analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (M01141-1)

Human, Monkey, Mouse, Rat

$370
Cat # M01141-1
100 μg/vial

Anti-14-3-3 Zeta (V52) YWHAZ Antibody

Western blot (WB) analysis of 14-3-3 ζ (V52) polyclonal antibody at 1:500 dilution
Lane1:Hela cell lysate
Lane2:Mouse brain tissue lysate
Lane3:Rat kidney tissue lysate

Immunohistochemistry (IHC) analyzes of 14-3-3 ζ (V52) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining

Immunohistochemistry (IHC) analyzes of 14-3-3 ζ (V52) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining

Human, Mouse, Rat

IF, IHC, WB

$405
Cat # A01141
100ul

Anti-14-3-3 Zeta (phospho-S58) YWHAZ Antibody

Western blot (WB) analysis of 14-3-3 ζ (phospho-S58) polyclonal antibody at 1:500 dilution
Lane1:The Brain tissue lysate of Mouse(40ug)
Lane2:The Brain tissue lysate of Rat(40ug)
Lane3:HEK293T treated with UV for 5 minutes then repair for 24 hours whole cell lysate(40ug)
Lane4:HEK293T treated with UV for 5 minutes then repair for 16 hours whole cell lysate(40ug)
Lane5:HEK293T whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of 14-3-3 ζ (phospho-S58) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining

Immunohistochemistry (IHC) analyzes of 14-3-3 ζ (phospho-S58) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining

Human, Mouse, Rat

IHC, WB

$482
Cat # A01141S58
100ul

Anti-Phospho-14-3-3 beta/zeta (S184/186) YWHAZ Antibody

Western blot analysis of lysates from RAW264

Human, Mouse, Rat

ELISA, WB

$380
Cat # A01141S184
100ul

Anti-Phospho-14-3-3 zeta/delta (T232) YWHAZ Antibody

Human, Mouse, Rat

ELISA, IF, IHC, WB

$380
Cat # A01141T232
100ul

14-3-3 zeta (YWHAZ) (NM_001135701) Human Recombinant Protein

$1249
Cat # PROTP63104
20 µg

Anti-YWHAZ Antibody

Fluorescent image of U251 cells stained with YWHAZ Antibody

Western blot analysis of lysates from A431, HepG2, mouse NIH/3T3, rat PC-12 and C6 cell line, mouse brain tissue (from left to right), using YWHAZ Antibody

Human, Mouse, Rat

IF, WB

$299
Cat # M01141-2
80 µl

14-3-3 zeta (YWHAZ) (NM_003406) Human Over-expression Lysate

14-3-3 zeta (YWHAZ) Human Over-expression Lysates NM_001135699

Human

$628
Cat # LS007534
20 µg, 100 µg

YWHAZ Overview

Facts about 14-3-3 protein zeta/delta.

14-3-3 protein zeta/delta (YWHAZ)

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif.

Binding generally results in the modulation of the activity of the binding partner. .

Gene Information

Human
Gene Name:	YWHAZ
Uniprot:	P63104
Entrez:	7534

Family

Belongs to:

14-3-3 family

Alternative Names

1433 zeta; 14-3-3 zeta; KCIP-1; KCIP-1MGC126532,14-3-3-zeta; MGC111427; MGC138156; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, deltapolypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zetapolypeptide; YWHAZ; zeta polypeptide

Molecular Weight

Mass (kDA):
27.745 kDA

Genomic Context


Human

Location:	8q22.3
Sequence:	8; NC_000008.11 (100916523..100954068, complement)

Subcellular Localizations

Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes.

Diseases

Malignant Neoplasms
Neoplasms
Carcinoma
Malignant Neoplasm Of Breast
Mammary Neoplasms
Malignant Paraganglionic Neoplasm
Neoplasm Metastasis
Nervousness
Cell Transformation, Neoplastic
Malignant Squamous Cell Neoplasm
Parkinson Disease
Head And Neck Neoplasms
Schizophrenia

Tissue Adhesions
Diabetes Mellitus
Inflammation
Endometrial Polyp
Leukemia
Mastitis
Pituitary Diseases

Pathways

Reverse Transcription
Protein Import
Pathogenesis
Cell Proliferation
Immune Response
Cell Differentiation
Transport
Translation
Embryo Development
Regeneration
Aging
Proteolysis
Regulation Of Gene Expression

Mrna Transcription
Localization
Cell Death
Autophagy
Cell Adhesion
Cytokinesis
Lymphocyte Proliferation

PTMs

Phosphorylation
Cleavage

Oxidation
Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/YWHAZ

References

PMID: 1577711 by Zupan L.A., et al. Cloning and expression of a human 14-3-3 protein mediating phospholipolysis. Identification of an arachidonoyl-enzyme intermediate during catalysis.

PMID: 9512661 by Seluja G.A., et al. Two unique 5' untranslated regions in mRNAs encoding human 14-3-3 zeta: differential expression in hemopoietic cells.