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- Table of Contents
SERPINA1 antibody (PB10097) performs well in WB and shows reactivity with Human, Monkey, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Western blot analysis of SERPINA1 using anti-SERPINA1 antibody (PB10097).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human T47D whole cell lysates,
Lane 2: monkey COS-7 whole cell lysates,
Lane 3: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINA1 antigen affinity purified polyclonal antibody (PB10097) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SERPINA1 at approximately 53 kDa. The expected band size for SERPINA1 is at 47 kDa.
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human T47D whole cell lysates,
Lane 2: monkey COS-7 whole cell lysates,
Lane 3: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINA1 antigen affinity purified polyclonal antibody (PB10097) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SERPINA1 at approximately 53 kDa. The expected band size for SERPINA1 is at 47 kDa.
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Anti-alpha 1 Antitrypsin/SERPINA1 Antibody Picoband®
SERPINA1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey, Mouse | Rabbit | WB |
Tested Applications
SERPINA1 antibody was positively detected by WB in:
human T47D whole cell, monkey COS-7 whole cell, mouse kidney tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Monkey, Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-alpha 1 Antitrypsin/SERPINA1 Antibody Picoband®
View all SERPINA1 Antibodies
View all SERPINA1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human SERPINA1 recombinant protein (Position: E25-T204). Human SERPINA1 shares 72.8% amino acid (aa) sequence identity with rat SERPINA1.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
53 kDa
Calculated Molecular Weight
46.7 kDa
Background of SERPINA1
SERPINA1 is also known as PI, A1A or AAT. This gene is mapped to 14q32.1. The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.
Cite This Product
Anti-alpha 1 Antitrypsin/SERPINA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10097)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
91%
High Homology
Rat
71%
Moderate Homology
Dog
71%
Moderate Homology
Sheep
69%
Low homology
Cow
69%
Low homology
Mouse
66%
Low homology
Rabbit
65%
Low homology
Zebrafish
42%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SERPINA1 antibody
Publications
Application:
Lv X, Gao F, Liu Q, Zhang S, Huang Z, Zhu Y, Zong H, Li Q, Li S. Exp Ther Med. 2018 Feb;15(2):1465-1473. doi: 10.3892/etm.2017.5554. Epub 2017 Nov 23. Clinical and pathological characteristics of IgG4-related interstitial lung disease
PMID: 29434730
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Product Promise
We promise Anti-alpha 1 Antitrypsin/SERPINA1 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB10097
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026