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Product Info Summary
| SKU: | M32338 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-ABRA1 Purified ABRAXAS1 Monoclonal Antibody
SKU/Catalog Number
M32338
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ABRA1 Purified ABRAXAS1 Monoclonal Antibody (Catalog# M32338). Tested in Flow Cytometry, IP, WB application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-ABRA1 Purified ABRAXAS1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M32338)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
ABRA1-01
Isotype
Mouse IgG2a kappa
Immunogen
Recombinant protein corresponding to amino acids 1-313 of ABRA1 with N-terminal His6 tag. The mouse monoclonal antibody ABRA1-01 recognizes human ABRA1 (Abraxas, CCDC98), an adaptor protein involved in DNA repair, which migrates as a 45 kDa band on PAAGE under reducing conditions.
Cross-reactivity
Cross-reactivity with LARGE2 was not determined.
Reactive Species
M32338 is reactive to ABRAXAS1 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
46.7 kDa
Background of ABRAXAS1
ABRA1 (Abraxas), also known as CCDC98, is an adaptor protein that is essential for formation and function of BRCA1 A tumor suppressor complex. This complex plays critical roles in DNA repair, cell cycle checkpoint control, and maintenance of genomic stability. ABRA1 mediates interaction of ubiquitin-interacting motif-containing protein RAP80 and deubiquitination enzyme BRCC36 with BRCA1/BARD1. ABRA1 controls both DNA-damage-induced formation of BRCA1 foci and BRCA1-dependent G2/M checkpoint activation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M32338 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-2 μg/ml. Intracellular staining.
Western blotting: Reducing conditions.
Validation Images & Assay Conditions
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Western blotting detection of ABRA1-EGFP fusion protein in lysate of transfected HEK293 cells.
Lane A: Immunostaining with anti-ABRA1 (clone ABRA1-01)
Lane B: Immunostaining with anti-EGFP (polyclonal)
Lane C: Immunostaining with anti-alpha tubulin (clone TU-01)
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Separation of MOLT-4 cells stained using anti-ABRA1 (ABRA1-01) purified antibody (concentration in sample 9 µg/ml, GAM APC, red-filled) from MOLT-4 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
Specific Publications For Anti-ABRA1 Purified ABRAXAS1 Monoclonal Antibody (M32338)
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