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Product Info Summary
| SKU: | M01645 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-Adenylate Kinase 1 (AK1) Mouse Monoclonal Antibody [Clone ID: OTI8A1]
SKU/Catalog Number
M01645
Size
100 µl
Description
Boster Bio AK1 (Adenylate Kinase 1) mouse monoclonal antibody, clone OTI8A1 (formerly 8A1). Catalog# M01645. Tested in FC, IF, WB. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Adenylate Kinase 1 (AK1) Mouse Monoclonal Antibody [Clone ID: OTI8A1] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01645)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI8A1
Isotype
IgG1
Immunogen
Full length human recombinant protein of human AK1 (NP_000467) produced in HEK293T cell.
Reactive Species
M01645 is reactive to AK1 in Human, Monkey, Mouse, Rat
Calculated molecular weight
21.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01645 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2500~5000
IF 1:100
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AK1 monoclonal antibody.
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HEK293T cells transfected with either AK1 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AK1 antibody (M01645)
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Anti-AK1 mouse monoclonal antibody (M01645) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AK1.
Specific Publications For Anti-Adenylate Kinase 1 (AK1) Mouse Monoclonal Antibody [Clone ID: OTI8A1] (M01645)
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