Product Info Summary
SKU: | A05022 |
---|---|
Size: | 2mL |
Reactive Species: | Human, Rabbit |
Host: | Goat |
Application: | IP, WB |
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Product info
Product Name
Anti-Aldolase ALDOA Antibody
View all Aldolase A Antibodies
SKU/Catalog Number
A05022
Size
2mL
Form
Lyophilized
Description
Boster Bio Anti-Aldolase ALDOA Antibody (Catalog # A05022). Tested in IP, WB applications. This antibody reacts with Human, Rabbit.
Storage & Handling
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-Aldolase ALDOA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05022)
Host
Goat
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
Aldolase [Rabbit Muscle]
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05022 is reactive to ALDOA in Human, Rabbit
Applications
A05022 is guaranteed for IP, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
39.42kDa
Background of Aldolase A
Aldolase plays a key role in glycolysis and gluconeogenesis. In addition, it may also function as scaffolding protein. In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in the liver, and aldolase C in the brain. Alkylation of Arg-43 inactivates the enzyme. Aldolase is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 2.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:20,000
IP: 1:100
WB: 1:500 - 1:5,000
Validation Images & Assay Conditions
Click image to see more details
Immunoprecipitationof rabbit anti Aldolase antiserum
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Figure 1. Western blot analysis of ALDOA using anti-ALDOA antibody (A05022).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDOA antigen affinity purified polyclonal antibody (Catalog # A05022) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Rabbit anti-Goat IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1023) with Tanon 5200 system. A specific band was detected for ALDOA.
Click image to see more details
Figure 2. Western blot analysis of ALDOA using anti-ALDOA antibody (A05022).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDOA antigen affinity purified polyclonal antibody (Catalog # A05022) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Rabbit anti-Goat IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1023) with Tanon 5200 system. A specific band was detected for ALDOA.
Protein Target Info & Infographic
Gene/Protein Information For ALDOA (Source: Uniprot.org, NCBI)
Gene Name
ALDOA
Full Name
Fructose-bisphosphate aldolase A
Weight
39.42kDa
Superfamily
class I fructose-bisphosphate aldolase family
Alternative Names
ALDA; aldolase A, fructose-bisphosphate; EC 4.1.2.13; fructose-1,6-bisphosphate triosephosphate-lyase; fructose-bisphosphate aldolase A; GSD12; Lung cancer antigen NY-LU-1; MGC10942; MGC17716; MGC17767; Muscle-type aldolase ALDOA ALDA, GSD12, HEL-S-87p aldolase, fructose-bisphosphate A fructose-bisphosphate aldolase A|aldolase A, fructose-bisphosphate|epididymis secretory sperm binding protein Li 87p|fructose-1,6-bisphosphate triosephosphate-lyase|lung cancer antigen NY-LU-1|muscle-type aldolase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALDOA, check out the ALDOA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALDOA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Aldolase ALDOA Antibody (A05022)
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