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NCOA4 antibody (A04368-3) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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Flow Cytometry analysis of HL-60 cells using anti-NCOA4 antibody (A04368-3).
Overlay histogram showing HL-60 cells stained with A04368-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NCOA4 Antibody (A04368-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HL-60 cells stained with A04368-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NCOA4 Antibody (A04368-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-NCOA4 Antibody Picoband®
NCOA4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
NCOA4 antibody was positively detected by WB in:
human 293T whole cell, human PC-3 whole cell, human Hela whole cell, human HL-60 whole cell, human HepG2 whole cell, human Daudi whole cell, human A431 whole cell, rat liver tissue, rat RH35 whole cell, mouse liver tissue, mouse HEPA1/6 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
NCOA4 antibody was positively detected by FC in:
HL-60 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-NCOA4 Antibody Picoband®
View all NCOA4 Antibodies
View all NCOA4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NCOA4 recombinant protein (Position: R14-M614).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
70 kDa
Calculated Molecular Weight
69.7 kDa
Background of NCOA4
Nuclear receptor coactivator 4 is a protein that in humans is encoded by the NCOA4 gene. This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14.
Cite This Product
Anti-NCOA4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04368-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
96%
High Homology
Cow
85%
Moderate Homology
Sheep
84%
Moderate Homology
Dog
84%
Moderate Homology
Mouse
81%
Moderate Homology
Rat
79%
Moderate Homology
Rabbit
76%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for NCOA4 antibody
Flow Cytometry (FC) protocol for NCOA4 antibody
Publications
Application:
Species:
Targeting Ncoa4-mediated ferritinophagy ameliorates arsenism by restoring redox homeostasis and suppressing ferroptosis
Published: 2026-08-28
Journal: Journal of Environmental Sciences
Application: WB
Impact Factor: 6.50
Species: Zebrafish
Ischemic Stroke Induces Ferroptosis and Neuroinflammation via Activation of the Microglial Tbp-Lpl Transcriptional Axis
Published: 2026-06-10
Journal: NEUROCHEMICAL RESEARCH
Application: WB
Impact Factor: 4.50
Species: Mouse
PMID: 42268517
Yishen Gushu formula exerts osteoprotective effects in OVX-Induced PMOP rats: role of ferroptosis Inhibition and iron metabolism correction
Published: 2026-02-04
Journal: HEREDITAS
Application: WB,IHC
Impact Factor: 2.50
Species: Rat
PMID: 41639935
Isorhynchophylline protects against ferroptosis in diabetic nephropathy by activating Nrf2
Published: 2025-09-01
Journal: NEFROLOGIA
Application: WB
Impact Factor: 2.60
Species: Mouse,Human
An in-depth analysis of the effects of excessive acetochlor exposure on chicken liver health
Published: 2024-12-27
Journal: PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
Application: WB
Impact Factor: 4.20
Species: Chicken
PMID: 40015872
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Product Promise
We promise Anti-NCOA4 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A04368-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026