Anti-beta-hCG Purified CGB3 Monoclonal Antibody

Product Name

Anti-beta-hCG Purified CGB3 Monoclonal Antibody

View all CGB Antibodies

SKU/Catalog Number

M13820

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-beta-hCG Purified CGB3 Monoclonal Antibody (Catalog# M13820). Tested in ICC, ELISA application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-beta-hCG Purified CGB3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13820)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

HCG-61

Isotype

Mouse IgG2b

Immunogen

Human chorionic gonadotropin. The antibody HCG-61 reacts with beta-subunit of human chorionic gonadotropin, a 40 kDa hormone. hCG belongs to the glycoprotein hormone family together with lutropin (LH), follitropin (FSH) and thyrotropin (TSH). hCG is synthesised by trophoblastic cells of the placenta during pregnancy and stimulates the growth of corpus luteum. The HCG-61 antibody reacts with association constant 5.1 x 10¹⁰ l/mol.

Cross-reactivity

Following cross-reactivity (%) was determined by classic double-antibody RIA using unlabelled hormones: beta-hCG (77), alpha-hCG (1.3), hLH (0.86), hFSH (< 0.5), hTSH (< 0.5).

Reactive Species

M13820 is reactive to CGB in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

17.7 kDa

Background of CGB

Choriogonadotropin (hCG) is a protein of the molecular weight about 40 kDa. It belongs to the glycoprotein hormone family together with lutropin (LH), follitropin (FSH) and thyrotropin (TSH). Choriogonadotropin is synthesised by trophoblastic cells of the placenta during pregnancy and stimulates the growth of corpus luteum. The other hormones are produced by anterior pituitary gland. All these hormones are structurally related, being composed of two noncovalently associated subunits alpha and beta.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M13820 is guaranteed for ELISA, ICC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry: 10 μg/ml; cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton-X100.
Western blotting: 1 μg/ml; positive control: murine brain lysate (red. Laemmli buffer).

Validation Images & Assay Conditions

See full target information CGB

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