|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-Cytokeratin 8 Antibody|
|Description||Rabbit IgG polyclonal antibody for Keratin, type II cytoskeletal 8(KRT8) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-Cytokeratin 8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1240)|
|Replacement Item||This antibody may replace the following items: sc-101459|sc-130312|sc-134484|sc-241376|sc-293099|sc-32328|sc-374027|sc-374274|sc-374275|sc-52324|sc-52325|sc-53266|sc-56376|sc-57004|sc-58736|sc-58737|sc-58825|sc-70928|sc-70939|sc-73480|sc-8018|sc-8020|sc-8021 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Cytokeratin 8(127-145aa QQQKTARSNMDNMFESYIN), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Keratin, type II cytoskeletal 8|
|Molecular Weight||53704 MW|
|Protein Function||Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. .|
|Tissue Specificity||Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. .|
|Sequence Similarities||Belongs to the intermediate filament family.|
|Subcellular Localization||Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix .|
|Alternative Names||Keratin, type II cytoskeletal 8;Cytokeratin-8;CK-8;Keratin-8;K8;Type-II keratin Kb8;KRT8;CYK8;|
|Research Areas|||signal transduction|cytoskeleton / ecm|cytoskeleton|intermediate filaments|class i|keratin||
Background for Keratin, type II cytoskeletal 8
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Cytokeratin 8 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Cytokeratin 8 Antibody Images
Click the images to enlarge.
Lane 1: HELA Cell Lysate
Lane 2: HT1080 Cell Lysate
Lane 3: COLO320 Cell Lysate
IHC(P): Human Mammary Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,