SKU MA1011
Size 100μg/vial
Reactivity Chicken, Human, Turkey
Clonality Monoclonal
Clone Number CP-93
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, ICC, WB

Overview

Product Name Anti-Calponin Antibody (Monoclonal, CP-93)
SKU/Catalog Number MA1011
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Calponin, calponin 2 (CNN2) detection. Tested with WB, IHC-P, ICC in Human;chicken;turkey. No cross reactivity with other proteins.
Cite This Product Anti-Calponin Antibody (Monoclonal, CP-93) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1011)
Host Mouse
Immunogen Turkey gizzard calponin
Reactivity Chicken, Human, Turkey

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, chicken, turkey, By Heat
Immunocytochemistry , 1μg/ml, Human, chicken, turkey, -
Western blot, 0.25-0.5μg/ml, Human, chicken, turkey

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q08093
Gene Name Cnn2
Protein Name Calponin-2
Tissue Specificity Smooth muscle, and tissues containing significant amounts of smooth muscle.
Alternative Names Calponin-2;Calponin H2, smooth muscle;Neutral calponin;Cnn2;
Molecular Weight 33156 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Background Calponin is a basic 34-kD protein first isolated from chicken gizzard and bovine aorta. It is specifically expressed in smooth muscle and binds calmodulin, actin, and tropomyosin. It is able to inhibit the ATPase activity of myosin and is though to play a role in smooth muscle contraction. Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein though to be involved in some way in the regulation or modulation of contraction.

Order Product (MA1011)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

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Publications

Liu C, Chen L, Zeng J, Cui J, Ning Jn, Wang Gs, Belguise K, Wang X, Qian Gs, Lu Kz, Yi B. Exp Cell Res. 2015 Aug 1;336(1):109-18. Doi: 10.1016/J.Yexcr.2015.05.025. Epub 2015 Jun 10. Bone Morphogenic Protein-2 Regulates The Myogenic Differentiation...

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.