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- Table of Contents
ENG antibody (A02997-3) performs well in ELISA, Flow Cytometry, IF, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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Flow Cytometry analysis of U87 cells using anti-CD105/ENG antibody (A02997-3).
Overlay histogram showing U87 cells stained with A02997-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD105/ENG Antibody (A02997-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U87 cells stained with A02997-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD105/ENG Antibody (A02997-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-CD105/ENG Antibody Picoband®
ENG antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IF, IHC, WB |
Tested Applications
ENG antibody was positively detected by WB in:
human SiHa whole cell, human Hela whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ENG antibody was positively detected by IHC in:
human bladder urothelial carcinoma tissue, human lung cancer tissue, human liver cancer tissue, human endometrial adenocarcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ENG antibody was positively detected by IF in:
human ovarian cancer tissue
ENG antibody was positively detected by FC in:
U87 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-CD105/ENG Antibody Picoband®
View all ENG Antibodies
View all ENG Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD105/ENG recombinant protein (Position: E26-H267).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
95 kDa
Calculated Molecular Weight
70.6 kDa
Background of ENG
Endoglin (Osler-Rendu-Weber syndrome 1), CD105, is a type I membrane glycoprotein located on cell surfaces and is a part of the TGF beta receptor complex. Its gene is mapped to human chromosome 8. The protein consists of a homodimer of 180 kDA with disulfide links. It has been found on endothelial cells, activated macrophages, fibroblasts and smooth muscle cells. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling and has been found to be elevated in pregnant women who subsequently develop preeclampsia.
Cite This Product
Anti-CD105/ENG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02997-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
93%
High Homology
Rabbit
71%
Moderate Homology
Dog
71%
Moderate Homology
Sheep
69%
Low homology
Cow
67%
Low homology
Mouse
66%
Low homology
Rat
66%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ENG antibody
Immunohistochemistry (IHC) protocol for ENG antibody
Immunofluorescence (IF) protocol for ENG antibody
Flow Cytometry (FC) protocol for ENG antibody
Publications
Application:
Species:
Targeted Retention of Cationic Liposomes at the Inflamed Blood–Brain Barrier by Incorporating Mesenchymal Stem Cell Membrane
Published: 2026-07-30
Journal: Advanced Healthcare Materials
Application: WB
Impact Factor: 11.00
Species: Human
PMID: 42533406
De Novo Transcriptome Analysis of Primed Canine Umbilical Cord Derived Mesenchymal Stem Cells Uncovers a Collection of Antimicrobial Peptides
Published: 2026-02-17
Journal: International Journal of Peptide Research and Therapeutics
Application: IF
Impact Factor: 2.40
Species: Canine
Precisely targeted drug delivery by mesenchymal stem cells-based biomimetic liposomes to cerebral ischemia-reperfusion injured hemisphere
Published: 2024-06-11
Journal: JOURNAL OF CONTROLLED RELEASE
Application: WB
Impact Factor: 10.50
Species: Human
PMID: 38851537
Canine umbilical cord tissue derived mesenchymal stem cells naturally express mRNAs of some antimicrobial peptides
Published: 2023-04-15
Journal: VETERINARY RESEARCH COMMUNICATIONS
Application: IF
Impact Factor: 2.20
Species: Dog
PMID: 37059874
Pre-vascularization in fibrin Gel/PLGA microsphere scaffolds designed for bone regeneration
Published: 2018-08-28
Journal: NPG Asia Materials
Application: IF
Impact Factor: 7.21
Species: Rabbit
Review
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Product Promise
We promise Anti-CD105/ENG Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A02997-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026