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Facts about Endoglin.
Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity).
Human | |
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Gene Name: | ENG |
Uniprot: | P17813 |
Entrez: | 2022 |
Belongs to: |
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No superfamily |
CD105 antigen; CD105; Endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1
Mass (kDA):
70.578 kDA
Human | |
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Location: | 9q34.11 |
Sequence: | 9; NC_000009.12 (127815012..127854773, complement) |
Detected on umbilical veil endothelial cells (PubMed:10625079). Detected in placenta (at protein level) (PubMed:1692830). Detected on endothelial cells (PubMed:1692830).
Cell membrane; Single-pass type I membrane protein.
Whether you're interested in using this gene for research or are looking for other uses, you've come to the right place. This article will provide details about the Angiogenic marker (ENG) as well as CD34. It will also provide the best applications for this gene. Additionally, you'll learn about Endoglin and CD105 and CD105, as well as other angiogenic markers.
The Angiogenic Marker (ATM) is a biomarker of tumor angiogenesis. It shares several similarities with other processes, such as cell-cycle and cell death, growth the process of migration, and growth. Angiogenesis is a process that involves multiple genes, such as AKT1, EGFR and IL6 PECAM1, PTGS2 and VEGF. Boster Bio's ATM offers an easy and efficient method to determine the expression of this gene.
Boster bio encodes the CD276 protein. It has many functions. It triggers NFKB signaling through TLR-4 , and promotes upregulation of VEGF and the IL-8. Increased levels of VEGF and IL-8 are vital for angiogenesis as well as tumor invasion and targeting this protein can aid in the development of new therapies for tumor angiogenesis. The CD276 antigen is not yet approved for human use.
Numerous studies have proven the function of the expression of vascular markers in cancer cells that are aggressive. The cells express VEGF and TIE2 in a manner similar as melanoma cell lines, making them angiogenic factors. Furthermore, bc48's upregulation is been linked with the development of vascular tubes within endothelial cell of the endothelial cell as well as the endothelin B receptor. These findings are promising for creation of novel therapies and diagnostics for breast cancer.
The informationgraphics of the Angiogenic marker of Boster offer a wealth of information about each gene. Boster offers gene informationgraphics for mouse and human. You can look up any gene of interest and view its Boster Bio profile. The Angiogenic Marker in Boster Bio features information about the gene with just a single click. So, what's the Angiogenic Marker in Boster Bio?
For this study, we used the 5-ethynyl-2'-deoxyuridine (EdU) Cell Proliferation assay kit. HUVECs were cultured in glass coverslips on sterile six-well plates. After the incubation process with 50 mM EdU and incubation in sterile 6-well plates. After incubating with EdU for two hours, HUVECs were washed twice with PBS. We then fixed the cells with 4% paraformaldehyde for 30 min at RT. Next, we permeated the cells with 0.5 percent Triton X100.
Antibodies against CD105/Endoglin (Angiogenesis Marker) react with human cells. This antibody has been validated in IHC, ICC, and WB on both negative and positive samples. The Boster Bio Anti-Endoglin/ CD105 antibody is available without BSA. You may also purchase blocking peptides with a concentration of 1.0 mg/ml.
Using a high-throughput RNA-seq platform A team of researchers has identified a variety of cells with varying levels of expression of Endoglin. The cell lines were sortable by increasing levels of Endoglin CpG methylation. The data were then analyzed by Kaplan-Meier and Cox regression techniques, and different functional traits were evaluated using a two-way and one-way analysis of variance. P-values that were less than 0.05 were considered significant.
Methylation studies have confirmed the Endoglin's methylation in lung cancer cell lines using Pyrosequencing and RNA sequencing. Western blotting analysis confirmed that methylation-dependent silencing of Endoglin significantly reduced protein levels in lung cancer cell lines. This methylation-dependent silencing was also confirmed using immunohistochemistry, and results are summarized in Figure 6D-G.
Endoglin is a member of the CD105 family, has been associated with human vascular endothelium. It is found in monocytes with activation and bone proerythroblasts in the marrow. It binds the transforming growth factor-beta (TGF-b) receptor complex. This protein plays a crucial role in cardiovascular development and remodeling. It is also controlled throughout the development of the heart.
Many tumors with abnormal gene expression levels can be detected by testing for Molecular Endoglin in cancer cells. It can also identify endoglinmethylation which is the sign of HER2-positive cancer. Moreover it is linked to a good prognosis in the early stages of cancer. This is a good thing for those who are looking for new methods to detect the protein.
Endoglin was found to be effective in reducing proliferation of cancerous cells. These studies have shown a reduction in the proliferation of colorectal and basal cell cancer cells. These findings suggest that a decreased proliferation of these tumors could be due to an increase in the activity of the cell-cycle inhibitor protein p16. The ENG marker has been used successfully to detect colon cancer in patients.
The expression levels of RPKM in Endoglin were evaluated using Microsoft Excel. The values were then compared with the Infinium probe beta values for site methylation. The values in both samples were very similar. The results showed that the methylation level of the endoglin gene was greater than that of the other gene, the b-actin gene.
The Anti-Endoglin/CD105 (Angiogenesis Marker) Monoclonal Antibody by Boster Bio has been tested against mouse and human. This antibody reacts with positive and negative samples, and is therefore non-hazardous for the lab. There are many kits of antibodies that don't contain BSA. To ensure the highest level of specificity and affinity, antibodies are tested against both positive and negative samples.
PMID: 8370410 by Bellon T., et al. Identification and expression of two forms of the human transforming growth factor-beta-binding protein endoglin with distinct cytoplasmic regions.
PMID: 1692830 by Gougos A., et al. Primary structure of endoglin, an RGD-containing glycoprotein of human endothelial cells.
*More publications can be found for each product on its corresponding product page