Skip to the beginning of the images gallery

Kit antibody (A01335-1) performs well in IHC, WB and shows reactivity with Human.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 18 Publications

a01335 1 kit primary antibodies ihc testing 2

IHC analysis of Kit using anti-Kit antibody (A01335-1).
Kit was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Kit Antibody (A01335-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Bundle Save

Bundle & Save Program

Save up to 50% on Picoband® antibodies + earn 10% back on eligible partner reagents.

View Details

BUY MORE, SAVE MORE

This antibody qualifies!
2 = 20% off · 3 = 30% off · 4 = 40% off · 5+ = 50% off

View Details

Free Secondary Antibody

How to redeem? While adding to cart, choose a size with a FREE Secondary Antibody!

View Details

Anti-c-Kit/Kit Antibody Picoband®

Kit antibody

18 Publications

Reactive Species	Host	Application
Human	Rabbit	IHC, WB

Tested Applications

Kit antibody was positively detected by WB in:

human HepG2 whole cell, human 293T whole cell, human K562 whole cell, human A549 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Kit antibody was positively detected by IHC in:

human mammary cancer tissue, human placenta tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Mouse

Predicted

Rat

Predicted

Product Information

Product Name

Anti-c-Kit/Kit Antibody Picoband®
View all KIT Antibodies

Form

Lyophilized

Uniprot

P05532

Gene ID

16590

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse c-Kit/Kit, which shares 88.6% and 94.3% amino acid (aa) sequence identity with human and rat c-Kit/Kit, respectively.

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

145 kDa

Calculated Molecular Weight

109.3 kDa

Background of Kit

SCFR (Mast/stem cell growth factor receptor), also known as KIT ONCOGENE or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.

Cite This Product

Anti-c-Kit/Kit Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01335-1)

Protocols

Western Blot protocol for Kit antibody

Review Protocol

Immunohistochemistry (IHC) protocol for Kit antibody

Review Protocol

Publications

Small bowel gastrointestinal stromal tumor: a retrospective study of 32 cases at a single center and review of the literature

Published: 2018-08-22

Journal: Therapeutics and Clinical Risk Management

Application: IHC

Impact Factor: 2.00

Species: Human

PMID: 30174429

Bundle Save

Bundle & Save Program

BUY MORE, SAVE MORE

Free Secondary Antibody

Anti-c-Kit/Kit Antibody Picoband®

Kit antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Small bowel gastrointestinal stromal tumor: a retrospective study of 32 cases at a single center and review of the literature

Identification and Distribution of the Interstitial Cells of Cajal in the Abomasum of Goats

Double sex and mab-3 related transcription factor 1 regulates differentiation and proliferation in dairy goat male germline stem cells

Ultrastructure damage of oviduct telocytes in rat model of acute salpingitis

Wnt1 Inhibits Hydrogen Peroxide-Induced Apoptosis in Mouse Cardiac Stem Cells

Ginsenosides promote proliferation of chicken primordial germ cells via PKC-involved activation of NF-κB

Embryonic hepatocyte transplantation for hepatic cirrhosis: Efficacy and mechanism of action

The inhibition of Endostar on the angiogenesis and growth of gastrointestinal stromal tumor xenograft

An immunohistochemical study of S-100 protein in the intestinal tract of Chinese soft-shelled turtle, Pelodiscus sinensis

Cancer stem-like cells can be isolated with drug selection in human ovarian cancer cell line SKOV3

Characterization of primary ovarian cancer cells in different culture systems

The expression of Cajal cells at the obstruction site of congenital pelviureteric junction obstruction and quantitative image analysis

Selective tropism of liver stem cells to hepatocellular carcinoma in vivo

Histological type of oncogenity and expression of cell cycle genes in tumor cells from human mesenchymal stem cells

Activation of protein kinases A and C promoted proliferation of chicken primordial germ cells

Stimulating effects of androgen on proliferation of cultured ovarian germ cells through androgenic and estrogenic actions in embryonic chickens

Effects of follicle-stimulating hormone and 17β-estradiol on proliferation of chicken embryonic ovarian germ cells in culture

Effects of follicle-stimulating hormone and androgen on proliferation of cultured testicular germ cells of embryonic chickens

Review

Have you used Anti-c-Kit/Kit Antibody Picoband®?

Product Promise

Troubleshooting

Boster Guarantee

Guaranteed product quality