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Product Info Summary
| SKU: | A01632 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC |
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Product info
Product Name
Anti-CD21 CR2 Monoclonal Antibody
SKU/Catalog Number
A01632
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD21 CR2 Monoclonal Antibody catalog # A01632. Tested in IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD21 CR2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01632)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
2C5
Isotype
IgG1
Immunogen
Synthetic Peptide of CD21
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01632 is reactive to CR2 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
112.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01632 is guaranteed for IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:200
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, CD21 Monoclonal Antibody (2C5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, CD21 Monoclonal Antibody (2C5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Immunofluorescence analysis of Mouse-liver tissue. 1, CD21 Monoclonal Antibody (2C5) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Specific Publications For Anti-CD21 CR2 Monoclonal Antibody (A01632)
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Customer Reviews
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7 Customer Q&As for Anti-CD21 CR2 Monoclonal Antibody
Question
Will anti-CD21 Monoclonal antibody A01632 work on primate IHC with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
Our lab technicians have not validated anti-CD21 Monoclonal antibody A01632 on primate. You can run a BLAST between primate and the immunogen sequence of anti-CD21 Monoclonal antibody A01632 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate leukemic t-cell in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-17
Question
I have attached the WB image, lot number and protocol we used for lymph using anti-CD21 Monoclonal antibody A01632. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-27
Question
I see that the anti-CD21 Monoclonal antibody A01632 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-02-26
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-02-26
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph using anti-CD21 Monoclonal antibody A01632. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-06-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-11
Question
Would A01632 anti-CD21 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-08-23
Answer
It shows on the product datasheet, A01632 anti-CD21 Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-23
Question
Is a blocking peptide available for product anti-CD21 Monoclonal antibody (A01632)?
Verified Customer
Verified customer
Asked: 2017-07-13
Answer
We do provide the blocking peptide for product anti-CD21 Monoclonal antibody (A01632). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-07-13
Question
Is there a BSA free version of anti-CD21 Monoclonal antibody A01632 available?
D. Baker
Verified customer
Asked: 2013-09-25
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD21 Monoclonal antibody A01632 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-09-25
