Anti-CTHRC1 Antibody

Product Name

Anti-CTHRC1 Antibody

View all CTHRC1 Antibodies

SKU/Catalog Number

A05203

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-CTHRC1 Antibody (Catalog # A05203). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

CTHRC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-CTHRC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05203)

Host

Rabbit

Contents

CTHRC1 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

CTHRC1 antibody was raised against a 19 amino acid peptide near the amino terminus of human CTHRC1. The immunogen is located within the first 50 amino acids of CTHRC1.

Cross-reactivity

CTHRC1 antibody is human, mouse and rat reactive. At least two isoforms of CTHRC1 are known to exist; this antibody will detect only the larger isoform.

Reactive Species

A05203 is reactive to CTHRC1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

26.2 kDa

Background of CTHRC1

The Collagen triple helix repeat-containing protein (CTHRC1) is a glycosylated, secreted protein that is transiently expressed in the arterial wall in response to injury where it is thought to contribute to vascular remodeling by inhibiting collagen expression and deposition and promoting cell migration (1). CTHRC1 is highly expressed multiple human cancers, including pancreatic cancer where it plays a significant role in the progression and metastasis of the disease by regulating migration and adhesion of tumors cells (2). CTHRC1 overexpression can also be used as an independent prognostic marker in gastric cancer (3). Mutations at this locus have been associated with Barrett esophagus and esophageal adenocarcinoma (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05203 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CTHRC1 antibody can be used for detection of CTHRC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information CTHRC1

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